Decision criteria for MALDI-TOF MS-based identification of filamentous fungi using commercial and in-house reference databases

Last updated on 1-10-2018 by Thibault Degiuli

Public Access

Published

Peer reviewed scientific article

English

Abstract:

BACKGROUND: Several Matrix-Assisted Laser Desorption/Ionization Time-of-Flight mass spectrometry protocols, which differ in identification criteria, have been developed for mold and dermatophyte identification. Currently, the most widely used approach is Bruker technology, although no consensus concerning the log(score) threshold has been established. Furthermore, it remains unknown how far increasing the number of spots to compare results might improve identification performance. In this study, we used in-house and Bruker reference databases as well as a panel of 422 isolates belonging to …

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