Determination of Bacillus cereus Emetic Toxin in Food Products by Means of LC-MS²

Last updated on 22-8-2019 by Anonymous (not verified)

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Peer reviewed scientific article


Cereulide is the heat-stable toxin produced by certain strains of Bacillus cereus. It is the main virulence factor of emetic B. cereus strains, which causes the emetic food poisoning syndrome, including rare fatal cases of food intoxications. Due to presumably low intoxication doses, a sensitive, specific, and robust technique is needed for its detection. In 2002, a LC-MS method was developed which allowed absolute quantification of cereulide using valinomycin as standard. This study describes the validation,according to the Commission Decision 2002/657/EC, of the LC-MS² method, a tandem ma…

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