Identification of the aadB gene coding for the aminoglycoside-2"-O-nucleotidyltransferase, ANT(2"), by means of the polymerase chain reaction36645

Last updated on 22-8-2019 by Anonymous (not verified)

Public Access


Peer reviewed scientific article



The polymerase chain reaction (PCR) was used to identify the gene encoding the aminoglycoside-2”-O-nucleotidyltransferase, ANT(2”). Two primers, delineating a DNA fragment of 188 bp, and a specific probe within this fragment were constructed, based on the nucleotide sequence of the aadB gene encoding this enzyme. Reference strains producing different aminoglycoside-modifying enzymes were used to evaluate the specificity and the sensitivity of the test. Strains producing the ANT(2”) enzyme showed the expected 188 bp DNA fragment after amplification. The oligonucleotide primers did not intera…

Associated health topics:

QR code

QR code for this page URL