Oxygenation activity of chicken blood phagocytes as measured by luminol- and lucigenin-dependent chemiluminescence8198

Last updated on 23-8-2019 by Anonymous (not verified)

Public Access

Published

Abstract:

Luminol- and lucigenin-dependent chemiluminescence (CL) was used to compare activation of the respiratory burst of chicken peripheral blood monocytes and heterophils after stimulation with various agents. Monocytes and heterophils were obtained from the blood of three specific-pathogen-free chickens at 14 months of age and purified by a two-step discontinuous Percoll gradient. All cells responded to phorbol 12-myristate 13-acetate (PMA), zymosan A and calcium ionophore A23187 producing CL. The time course of luminol- and lucigenin-dependent CL was similar for both monocytes and heterophils …

Associated health topics:

QR code

QR code for this page URL