Simulation of between repeat variability in real time PCR reactions.

Last updated on 23-8-2019 by Anonymous (not verified)

Peer reviewed scientific article

SCIENSANO

Abstract:

While many decisions rely on real time quantitative PCR (qPCR) analysis few attempts have hitherto been made to quantify bounds of precision accounting for the various sources of variation involved in the measurement process. Besides influences of more obvious factors such as camera noise and pipetting variation, changing efficiencies within and between reactions affect PCR results to a degree which is not fully recognized. Here, we develop a statistical framework that models measurement error and other sources of variation as they contribute to fluorescence observations during the amplific…

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