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Lymph node targeting of BCG vaccines amplifies CD4 and CD8 T-cell responses and protection against Mycobacterium tuberculosis34032

subcutaneous vaccination with 2x10(5) CFU BCG conferred only a transient protection. Hence, direct administration of M. bovis BCG to lymph nodes demonstrates that efficient targeting to lymph nodes may help to ...

Infectivity of rabies virus-exposed macrophages.

vitro, Mf4/4 spleen macrophages supported mild virus production (10(4)-fold less than neuroblastoma), with formation of typical virions. Bone marrow-derived macrophages (BMM) were most efficient to capture ...

New SYBR®Green methods targeting promoter sequences used for screening of several GM events pending for authorisation in Europe

The two methods allow a specific and sensitive detection of the targets in food and feed samples and can be used efficiently in different laboratories. Service:  Activités transversales en génomique ...

Bayesian estimation of the true prevalence, sensitivity and specificity of the Rose Bengal and indirect ELISA tests in the diagnosis of bovine brucellosis.

Specificity Serologic Tests Serotyping Abstract: Serology is the most convenient method for detecting brucellosis but the efficient use of such tests in disease control requires evaluation of diagnostic ...

Different replication profiles in specific-pathogen-free chickens of two H7 low pathogenic avian influenza viruses isolated from wild birds.

specific-pathogen-free (SPF) chickens, but poultry can be at risk from both. Indeed, the H7N1 isolated from the common shelduck had the ability to infect and to replicate efficiently in SPF chickens as indicated by high ...

Towards a Pathogenic Escherichia coli Detection Platform Using Multiplex SYBR(R)Green Real-Time PCR Methods and High Resolution Melting Analysis418

methods facilitate the detection of VTEC in a new highly efficient way and could represent the basis for developing a simple pathogenic E. coli platform Service:  Activités transversales en génomique ...

Clean up strategy for the analysis of PAH in food of animal origin

clean-up. In terms of the solvent consumption and clean-up efficiency, DACC demonstrated better performances and was therefore kept in the protocol. Then, different types of SPE were tested as a post DACC ...

Simulation of between repeat variability in real time PCR reactions.

influences of more obvious factors such as camera noise and pipetting variation, changing efficiencies within and between reactions affect PCR results to a degree which is not fully recognized. Here, we ...

Selection and characterization of a candidate therapeutic bacteriophage that lyses the Escherichia coli O104:H4 strain from the 2011 outbreak in Germany.

efficiently lyse the E. coli O104: H4 outbreak strain could be selected rather easily from a phage bank or isolated from the environment. It is argued that phage therapy should be more considered as a potential ...

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