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Genomic epidemiology of emerging ESBL-producing Kentucky in Europe.

which further spread has occurred. Comparative genomics indicated that the gene is present on the bacterial chromosome, within the type VI secretion system region. The gene is integrated downstream of the ...

Development of an NGS-Based Workflow for Improved Monitoring of Circulating Plasmids in Support of Risk Assessment of Antimicrobial Resistance Gene Dissemination.

localized on plasmids can be easily transferred between bacterial isolates by horizontal gene transfer, thereby contributing to the spread of AMR. Next-generation sequencing (NGS) technologies are ideal for ...

Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.

(EIEC) are responsible for millions of episodes of bacterial dysenteriae worldwide. Given its distinct epidemiology and public health relevance, only Shigellae are subject to mandatory reporting and ...

Transcriptional profiling of a laboratory and clinical Mycobacterium tuberculosis strain suggests respiratory poisoning upon exposure to delamanid

anti-tuberculous drug from the same class. Although delamanid is found to inhibit cell wall synthesis, the expression of genes involved in this process were only mildly affected. In contrast, a clear parallel was ...

RNA-based drug susceptibility testing of Mycobacterium tuberculosis

performed directly on crude cell lysate using a combination of the bead-based MagPixTM (Luminex) and QuantigeneTM Plex (Thermo Fischer) technology. Normalized, relative genes expression levels (control vs ...

Prediction of Susceptibility to First-Line Tuberculosis Drugs by DNA Sequencing

and drug susceptibility were identified across nine genes, and individual phenotypes were predicted unless mutations of unknown association were also present. To identify how whole-genome sequencing ...

Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:.

PLoS One, Volume 13, Issue 2 (2018) Keywords: bacterial phylogeny; food-borne; genes; NGS; WGS; computational biology/bioinformatics; disease outbreaks; outbreak; performance evaluation; pipeline; ...

Genetic elements associated with antimicrobial resistance among avian pathogenic Escherichia coli.

Anti-Bacterial Agents Chickens Drug Resistance, Bacterial Escherichia coli Escherichia coli Infections Escherichia coli Proteins Integrons Poultry Diseases Abstract: BACKGROUND: Avian-pathogenic Escherichia coli ...

High coverage metabolomics analysis reveals phage-specific alterations to Pseudomonas aeruginosa physiology during infection.

bacterial metabolism remains scarce. In this study, we tracked the dynamics of intracellular metabolites using untargeted high coverage metabolomics in Pseudomonas aeruginosa cells infected with lytic ...

Development of Luminex assays for identification of antimicrobial resistance genes.

resistance Development identification Luminex resistance Service:  Bacterial diseases Maladies bactériennes Bacteriële ziekten Category:  C32 ...

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