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Targeted Whole Genome Sequencing of the Capripoxvirus Genome from Clinical Tissue Samples and Lyophilized Vaccine Batches.

achieved by whole-genome sequencing. In this chapter, we describe a robust, cost-effective, and widely applicable protocol for reconstructing (nearly) complete CaPV genomes directly from clinical samples or ...

First Belgian Report of Ertapenem Resistance in an ST11 Klebsiella Pneumoniae Strain Isolated from a Dog Carrying blaSCO-1 and blaDHA-1 Combined with Permeability Defects

(MLST) and the identification and localization of antimicrobial resistance (AMR) and virulence-associated genes. Three plasmids were reconstructed from the whole-genome sequence (WGS) data: the conjugative ...

Identification of diffusion routes of O/EA-3 topotype of foot-and-mouth disease virus in Africa and Western Asia between 1974 and 2019- a phylogeographic analysis.

evolutionary phylodynamics of the causative serotype O viral strains all belonging to the East-Africa 3 topotype (O/ EA-3). We analyzed a total of 489 sequences encoding the FMDV VP1 genome region generated from ...

Outbreaks of Foot-and-Mouth Disease in Burundi, East Africa, in 2016, Caused by Different Serotypes.

estimated at 87%. Antibodies against FMDV serotypes O (52%), A (44%), C (19%), SAT1 (36%), SAT2 (58%), and SAT3 (23%) were detected across the provinces. FMDV genome was detected in samples from five of the ...

Orbivirus Screening from Imported Captive Oryx in the United Arab Emirates Stresses the Importance of Pre-Import and Transit Measures

Emirates. BTV-2 genome was detected in 6/16 of the Arabian Oryx, and amongst those, one out of six was seronegative. None of the tested samples was found positive for EHDV. Our results illustrate the wide ...

A robust, cost-effective and widely applicable whole-genome sequencing protocol for capripoxviruses

Publication Type: Peer reviewed scientific article Authors: Elisabeth Mathijs; Andy Haegeman; Kris De Clercq; Steven Van Borm; Frank Vandenbussche Source: Journal of Virological Methods (2022) Health Topics:  Animal health Santé animale Diergezondheid Ful ...

Metagenomic sequencing determines complete infectious bronchitis virus (avian Gammacoronavirus) vaccine strain genomes and associated viromes in chicken clinical samples.

genome with 99.95% nucleotide identity to vaccine strain 793B. In addition, substantial read counts (16% of viral reads) allowed the assembly of a near-complete chicken astrovirus genome, while lower read ...

Case Report: Multidrug Resistant Raoultella ornithinolytica in a Septicemic Calf

potentiated sulphonamides, streptomycin, tetracyclines and intermediate susceptibility for enrofloxacin. Whole genome sequencing confirmed identification as and the multidrug resistant character of the isolate. ...

Nearly Complete Genome Sequences of Two Bluetongue Viruses Isolated during the 2020 Outbreak in the Grand Duchy of Luxembourg.

virus serotype 8 (BTV-8) has been considered enzootic in France since 2018. Here, we report the nearly complete genome sequences of two BTV-8 isolates from the 2020 outbreak in the Grand Duchy ...

Development and evaluation of a core genome multilocus sequence typing scheme for Paenibacillus larvae, the deadly American foulbrood pathogen of honeybees

Publication Type: Peer reviewed scientific article Authors: Bertolotti, Alicia C.; Forsgren, Eva; Schäfer, Marc O.; EuroPLarva Consortium; Sircoulomb, Fabrice; Gaïani, Nicolas; Ribière‐Chabert, Magali; Paris, Laurianne; Lucas, Pierrick; de Boisséson, Clai ...

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