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Using a combination of short- and long-read sequencing to investigate the diversity in plasmid- and chromosomally encoded extended-spectrum beta-lactamases (ESBLs) in clinical Shigella and Salmonella isolates in Belgium

Publication Type: Peer reviewed scientific article Authors: Berbers, Bas; Vanneste, Kevin; Roosens, Nancy H. C. J.; Marchal, Kathleen; Pieter-Jan Ceyssens *; De Keersmaecker, Sigrid C. J. Source: Microbial Genomics, Volume 9, Issue 1 (2023) Health Topics: ...

A genomic appraisal of invasive Salmonella Typhimurium and associated antibiotic resistance in sub-Saharan Africa.

ST313- L2 likely emerged in the Democratic Republic of Congo around 1980 and further spread in the mid 1990s. We observed plasmid-borne as well as chromosomally encoded fluoroquinolone resistance ...

A global genomic analysis of Salmonella Concord reveals lineages with high antimicrobial resistance in Ethiopia.

demonstrate the presence of AMR markers integrated in structurally diverse IncHI2 and IncA/ C2 plasmids, and/or the chromosome. Molecular surveillance of pathogens such as S. Concord supports the understanding ...

First Belgian Report of Ertapenem Resistance in an ST11 Klebsiella Pneumoniae Strain Isolated from a Dog Carrying blaSCO-1 and blaDHA-1 Combined with Permeability Defects

ertapenem, third-generation cephalosporins and fluoroquinolones. A hybrid approach, combining long- and short-read sequencing, was employed for in silico plasmid characterization, multi-locus sequence typing ...

Genomic epidemiology of persistently circulating MDR Shigella sonnei strains associated with men who have sex with men (MSM) in Belgium (2013–19)

various incompatibility (Inc)-type plasmids, the SpA plasmid and ESBL  genes. CONCLUSIONS: In Belgium, S. sonnei isolates from men are much more likely to be resistant to important first-line antibiotics ...

Genomic epidemiology of emerging ESBL-producing Kentucky in Europe.

a 2854 bp plasmid fragment containing also IS. This is the first report of a chromosomally integrated CTX-M gene in spp. in Europe, previous studies having identified similar genes only on plasmids. Health ...

First detection of a plasmid located carbapenem resistant blaVIM-1 gene in E. coli isolated from meat products at retail in Belgium in 2015

Publication Type: Peer reviewed scientific article Authors: Cristina Garcia-Graells; Bas Berbers; Bavo Verhaegen; Kevin Vanneste; Marchal, Kathleen; Nancy Roosens; N Botteldoorn; Sigrid C.J. De Keersmaecker Source: International Journal of Food Microbiolo ...

Public microbial resources centres: key hubs for FAIR microorganisms and genetic materials.

microbiology, microbial domain biological resource centres (mBRCs) have a long-standing experience in preserving and distributing authenticated microbial strains and genetic materials (e.g., recombinant plasmids ...

Detection of Plasmid-Mediated Colistin Resistance, mcr-1 and mcr-2 Genes, in Salmonella spp. Isolated from Food at Retail in Belgium from 2012 to 2015.

mcr-2 determinant has been confirmed by whole genome sequencing and allowed the localization of these two genes on IncX4 type plasmids. We report here the presence of mcr-1 and the first mcr-2 gene in ...

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