Search results - 22 results
Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:.
Salmonella; Salmonella enterica; Salmonella typhimurium; SNP; subtyping; whole genome sequencing Abstract: Whole genome sequencing represents a promising new technology for subtyping of bacterial pathogens. ...
How can we better detect unauthorized GMO in the food and feed chain
6, Number 517 (2017) Keywords: detection DNA walking Monitoring new workflow next-generation-sequencing qPCR unauthorized GMO Abstract: Current GMO detection systems have limited abilities to detect ...
An integrated strategy combining DNA walking and NGS to detect GMO
Volume 232, Number 358 (2017) Keywords: detection DNA walking GMO Next-generation sequencing qPCR Abstract: Recently, we developed a DNA walking system for the detection and characterization of a broad ...
Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.
Techniques Gram-Negative Bacteria Humans Multiplex Polymerase Chain Reaction RNA, Ribosomal, 16S Abstract: OBJECTIVES: The objective of this study was to design and validate a genotyping method for multiplex ...
The added value of whole genome sequencing for the development of a specific detection method for an EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin
the impact of Whole Genome Sequencing (WGS) on food safety management: within a one health framework, NA, Issue FAO/GMI, NA (2016) Keywords: a an Bacillus Bacillus subtilis detection detection method ...
The use of whole genome sequencing (WGS) for typing of bacterial pathogenic isolates
Genome ON pathogenic Societies Society use WGS whole genome whole genome sequencing Health Topics: Food consumption and food safety Consommation et sécurité alimentaires Voedselconsumptie en –veiligheid ...
Strategies to detect unauthorized GMO in the food and feed chain (PhD thesis)
https://www.sciensano.be/en/biblio/strategies-detect-unauthorized-gmo-food-and-feed-chain-phd-thesis
pCAMBIA), were selected to develop a strategy allowing to detect and characterize a broad range of GMO. This strategy, fully integrated in the GMO routine analysis, consists to characterize sequences ...
Data analysis associated with the use of whole genome sequencing for subtyping of pathogenic microorganisms, Salmonella Typhimurium as a case study
sequencing Abstract: As the cost of bacterial whole genome sequencing (WGS) by NGS continues to decline, its application for routine (sub)typing of pathogenic microorganisms quickly becomes a reality. NGS ...
Current and new approaches in GMO detection: challenges and solutions
https://www.sciensano.be/en/biblio/current-and-new-approaches-gmo-detection-challenges-and-solutions
(e.g., DNA walking and Next Generation Sequencing (NGS)) or unknown (e.g., NGS) GMO. The benefits and drawbacks of these methods are discussed in this review. Health Topics: Food consumption and food ...
Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices.
DNA walking methods, anchored on the sequences used for the p35S or tNOS qPCR screening, were tested on Bt rice that contains these two transgenic elements. Second, the methods were assessed on a maize ...