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Evaluation of an interferon-gamma release assay for early detection of lumpy skin disease virus infection and vaccination in cattle.
Heat-inactivated antigen proved to be the stimulus of choice for LSD IGRA, providing increased sensitivity of the test and allowing its performance under BSL2 conditions. Under several conditions, LSDV IGRA showed ...
Development and Validation of a New DIVA Real-Time PCR Allowing to Differentiate Wild-Type Lumpy Skin Disease Virus Strains, Including the Asian Recombinant Strains, from Neethling-Based Vaccine Strains.
other capripox viruses was observed under field conditions in non-capripox viral stocks and negative animals. The high analytical sensitivity is translated into a high diagnostic specificity as more than ...
Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida (Coleoptera: Nitidulidae): A European Inter-Laboratory Comparison.
Laboratories in Europe participated in the study and analysed 12 samples with adult coleopterans and insect larvae. The performance of the laboratories was evaluated in terms of sensitivity and specificity ...
Selection and use of reference panels: a case study highlighting current gaps in the materials available for foot and mouth disease.
are required. These are to: reduce bias in estimates of the diagnostic sensitivity and inter-serotypic specificity of tests used to detect diverse strains of FMD virus (FMDV), provide bio-safe positive ...
Selection and use of reference panels: a case study highlighting current gaps in the materials available for foot and mouth disease
an example, this review highlights four specific areas where new reference reagents are required. These are to: reduce bias in estimates of the diagnostic sensitivity and inter-serotypic specificity of ...
Overview of diagnostic tools for Capripox virus infections.
purpose. The need for high throughput, high specificity/sensitivity and the capability for differentiating field virus strains from vaccine strains drives the development of new and better assays preferably ...
Evaluation of a commercial exogenous internal process control for diagnostic RNA virus metagenomics from different animal clinical samples
Virological Methods, Volume 283 (2020) Keywords: Animals Feces High-Throughput Nucleotide Sequencing Mengovirus Metagenomics Rna RNA Virus Infections RNA Viruses Swine Swine Diseases Viral Sensitivity and ...
Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.
Keywords: 5' Untranslated Regions Animals DNA Primers Foot-and-Mouth Disease Foot-and-Mouth Disease Virus Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Sensitivity ...
Evaluation of 16 commercial antibody ELISAs for the detection of bovine viral diarrhea virus-specific antibodies in serum and milk using well-characterized sample panels.
analytical sensitivity (DS e and AS e, respectively), diagnostic specificity (DS p), and repeatability relative to the virus neutralization (VN) test considered to be the gold standard assay. Using serum as ...
Characterization of three commercial ELISA kits for detection of BOHV-1 gE specific antibodies in serum and milk samples and applicability of bulk milk for determination of herd status.
a major pathogen of cattle. The diagnostic sensitivity (DS e) and specificity (DS p) of three commercial gE ELISA kits from IDEXX, IDV et and CIV- HIPRA were compared for serum and milk matrices. Limiting ...