Search results - 5 results
False-positive results in metagenomic virus discovery: a strong case for follow-up diagnosis.
Keywords: Animals Cattle Cattle Diseases DNA, Viral False Positive Reactions Female Metagenomics Parvoviridae Infections Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction ...
Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.
become the method of choice for routine viral diagnosis. As false-negative test results can have serious implications; an internal/external control system should be incorporated in each analysis to detect ...
A proposed validation method for automated nucleic acid extraction and RT-qPCR analysis: an example using Bluetongue virus.
Transcriptase Polymerase Chain Reaction Validation Studies as Topic Abstract: This study proposes a validation strategy for an automated extraction procedure, followed by RT-qPCR analysis. To avoid false-negative ...
A universal avian endogenous real-time reverse transcriptase-polymerase chain reaction control and its application to avian influenza diagnosis and quantification.
polymerase successfully reverses PCR inhibition. Second, an excellent strategy for detecting false negative samples is the coamplification of an internal control from each sample. We developed a universal ...
Evaluation of the enzyme-linked immunosorbent assay for the rapid screening and detection of classical swine fever virus antigens in the blood of pigs.
p.677-89 (1995) Keywords: African Swine Fever African Swine Fever Virus Animals Antigens, Viral Enzyme-Linked Immunosorbent Assay Evaluation Studies as Topic False Negative Reactions Sensitivity and ...