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Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
sample preparation methods allowed to obtain a full characterization to strain level of the spiked strain in the beef sample, carrying another non-pathogenic strain of E.coli. The simplest protocol was ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
improves the reconstruction of genomes and helps to resolve complex genomic contexts, especially in the case of metagenome analysis. To take the best advantage of long-read sequencing, DNA extraction methods ...