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Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling

adaptive sampling, following whole genome amplification to increase the amount of genetic material to meet sequencing standards. The strain-level data analysis was performed as previously described (Buytaers ...

RNA sequencing of avian paramyxovirus (Paramyxoviridae, Avulavirinae) isolates from wild mallards in Belgium, 2021: complete genomes and coinfections.

RNA sequencing to characterize three Avulavirinae isolates from pooled samples obtained from wild mallards in Belgium in 2021. The complete genome sequences of two avian Orthoavulavirus-1 (AOAV-1) ...

Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety

have always been analyzed and characterized after isolation. Since the advent of second generation sequencing, it has been possible to characterize the complete genome of these isolates to the SNP level. ...

ESCAIDE 2022 Oral presentation: International outbreak of Salmonella Typhimurium linked to a chocolate factory in 2022: Belgian findings

confirmed cases were identified using ECDC case definitions. Case-interviews focused on exposure to chocolate products of the concerned brand. Raw materials and finished food products collected at the factory ...

The African swine fever virus isolate Belgium 2018/1 shows high virulence in European wild boar.

genome were also detected in genitals and accessory sex glands of two boars. There were no antibodies detectable in commercial antibody ELISA s, antibody LFD s and indirect immunoperoxidase tests. Thus, ...

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