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Transforming Shiga toxin-producing surveillance through whole genome sequencing in food safety practices.
Abstract: INTRODUCTION: Shiga toxin-producing (STEC) is a gastrointestinal pathogen causing foodborne outbreaks. Whole Genome Sequencing (WGS) in STEC surveillance holds promise in outbreak prevention and ...
Validation strategy of a bioinformatics whole genome sequencing workflow for Shiga toxin-producing Escherichia coli using a reference collection extensively characterized with conventional methods
bacterial pathogenic isolates at single nucleotide resolution, making it the ultimate tool for routine surveillance and outbreak investigation. The lack of standardization, and the variation regarding ...
Strain-Level Metagenomic Data Analysis of Enriched In Vitro and In Silico Spiked Food Samples: Paving the Way towards a Culture-Free Foodborne Outbreak Investigation Using STEC as a Case Study.
simulated metagenomic data to explore the possibilities and limitations of this data analysis approach. The performed analysis allowed us to link the pathogenic strains from food samples to human isolates ...
A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine.
outbreak depends on the rapid and accurate identification of the responsible food source. Conventional methods based on isolation of the pathogen from the food matrix and target-specific real-time polymerase ...
The Benefits of Whole Genome Sequencing for Foodborne Outbreak Investigation from the Perspective of a National Reference Laboratory in a Smaller Country.
whole genome sequencing Abstract: Gradually, conventional methods for foodborne pathogen typing are replaced by whole genome sequencing (WGS). Despite studies describing the overall benefits, National ...
Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates
extraction, are required to ensure robust and exchangeable WGS data. Data sharing between (inter)national laboratories is essential to support foodborne pathogen control, including outbreak investigation. This ...
Global and regional source attribution of Shiga toxin-producing Escherichia coli infections using analysis of outbreak surveillance data
Topics: Pathogene E. coli Pathogenic E. coli E. coli pathogènes Service: Information sanitaire Gezondheidsinformatie Health information Manuscript versions: DOI: https://doi.org/10.1017/S095026881900116X ...
Associating sporadic, foodborne illness caused by Shiga toxin-producing Escherichia coli with specific foods: a systematic review and meta-analysis of case-control studies
Volume 147 (2019) Health Topics: Pathogenic E. coli Pathogene E. coli E. coli pathogènes Service: Information sanitaire Gezondheidsinformatie Health information Manuscript versions: DOI: ...
Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates.
C.J. De Keersmaecker Source: Sci Rep, Volume 10, Issue 1 (2020) Keywords: NGS pathogenic E. coli STEC WGS Abstract: Whole genome sequencing (WGS) has proven to be the ultimate tool for bacterial isolate ...