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Evaluation of an interferon-gamma release assay for early detection of lumpy skin disease virus infection and vaccination in cattle.

Heat-inactivated antigen proved to be the stimulus of choice for LSD IGRA, providing increased sensitivity of the test and allowing its performance under BSL2 conditions. Under several conditions, LSDV IGRA showed ...

Targeted Whole Genome Sequencing of the Capripoxvirus Genome from Clinical Tissue Samples and Lyophilized Vaccine Batches.

of tiled amplicons. The resulting amplicons can be sequenced on all currently available high-throughput sequencing platforms. As an example, we have included a detailed protocol for performing nanopore ...

Genomic comparison between and and analysis of peptide-based biomarkers for serodiagnosis.

to be potential antigens. As synthetic peptides are less costly and variable than recombinant proteins, an approach was performed to identify linear and discontinuous B-cell epitopes in the selected ...

PRRSV-Vaccinated, Seronegative Sows and Maternally Derived Antibodies (II): Impact on PRRSV-1 Vaccine Effectiveness and Challenge Outcomes in Piglets.

performed at weaning at three weeks of age, and all study piglets were challenged with a high dose of the PRRSV-1 07V063 strain at 6 weeks of age. A clear interference of MDA s was observed in the E+ piglets: ...

First Belgian Report of Ertapenem Resistance in an ST11 Klebsiella Pneumoniae Strain Isolated from a Dog Carrying blaSCO-1 and blaDHA-1 Combined with Permeability Defects

multidrug-resistant (MDR)  K. pneumoniae   ST11 carrying  blaSCO-1  and  blaDHA-1, isolated from a four-month-old dog in Belgium. Antimicrobial susceptibility testing (AST) of the isolate, performed via broth ...

Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida (Coleoptera: Nitidulidae): A European Inter-Laboratory Comparison.

Laboratories in Europe participated in the study and analysed 12 samples with adult coleopterans and insect larvae. The performance of the laboratories was evaluated in terms of sensitivity and specificity. ...

Selection and use of reference panels: a case study highlighting current gaps in the materials available for foot and mouth disease.

that can be used during assay development and in validation exercises to compare the performance of these varied (and sometimes competing) diagnostic technologies. World Organisation for Animal Health ...

Bluetongue Virus Infections in Cattle Herds of Manabí Province of Ecuador

for the first time, the presence of active BTV infections in Manabí province. We collected 430 serum samples from 38 randomly selected farms between March and July 2019 to perform BTV competitive ELISA. ...

Selection and use of reference panels: a case study highlighting current gaps in the materials available for foot and mouth disease

tests or kits provided by the commercial sector. Reference panels are essential tools that can be used during assay development and in validation exercises to compare the performance of these varied (and ...

Dietary exposure assessment and risk characterization of citrinin and ochratoxin A in Belgium

in three age categories (3–9, 10–17 and 18–64 years), obtained from a national food consumption survey. Furthermore, a risk characterization was performed for CIT, in which no intake values exceeded ...

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