Search results - 7 results
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
must be able to isolate pure high molecular weight (HMW) DNA from complex metagenomics samples, without introducing any bias. New methods released on the market, and protocols developed at the research ...
Growth of Stressed Strains of Four Non-O157 Shiga Toxin-Producing Escherichia coli Serogroups in Five Enrichment Broths.
(STEC) serogroups (O26, O103, O111, and O145) exposed to different stress conditions and (ii) the growth dynamics of stressed and nonstressed non- O157 STEC cells in five enrichment media. STEC strains ...
In vitro model to study the endocrine disrupting activity of migration products from plastic food contact materials
the cells exposed to it (after addition of luciferin, the substrate of luciferase). In a preliminary step, we first screened pure substances which were shown to migrate from plastic baby bottle, in ...
Characterization of Escherichia coli from raw poultry in Belgium and impact on the detection of Campylobacter jejuni using Bolton broth36828
Preston broth for enrichment and reliable detection of Campylobacter jejuni (both healthy and freeze stressed cells) was performed. Tested as pure cultures, Bolton broth enabled faster resuscitation and ...
Quantification of Campylobacter spp. in chicken carcass rinse by real-time PCR36795
pure DNA. The amplification efficiency was 100% and the square regression coefficient (R(2)) was 0.998. Quantification was linear over at least 7 log units. Using a crude cell lysate gave the highest ...
Purification, characterization and identification of a 32 kDa protein antigen of Mycobacterium bovis BCG36625
interferon in cultured spleen cells from BCG-sensitized mice. Moreover, P32 either pure or as part of BCG soluble extract promoted substantial antibody levels when injected in rabbits Service: Bacterial ...
The Enrichment of Histomonas meleagridis and Its Pathogen-Specific Protein Analysis: A First Step to Shed Light on Its Virulence.
of Histomonas meleagridis in 1893, the necessity of isolating pure H. meleagridis has been highlighted over the years in the battle against histomonosis. Insights into the molecular characteristics of ...