Search results - 173 results
Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking.
Publication Type: Peer reviewed scientific article Authors: Marie-Alice Fraiture; U Marchesi; D Verginelli; N. Papazova; Nancy Roosens Source: International Journal of Food Microbiology, Volume 354 (2021) Full text language: English Category: Peer-revie ...
Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking
Microbiology, Volume 354 (2021) Keywords: Antimicrobial resistance genes DNA walking anchored on pUB110 food and feed safety Genetically modified bacteria Protease real-time PCR detection Abstract: A PCR ...
Detection of Clinical and Subclinical Lumpy Skin Disease Using Ear Notch Testing and Skin Biopsies.
subclinical, the sensitivity of clinical surveillance could be lower than expected. Furthermore, real-time PCR for the detection of LSD viral DNA in blood samples from subclinical animals is only intermittently ...
Validation of TaqMan-Based Assays for Specific Detection and Differentiation of Wild-Type and Neethling Vaccine Strains of LSDV.
Petrović; Ilse De Leeuw; Andy Haegeman Source: Microorganisms, Volume 9, Issue 6 (2021) Keywords: differentiation DIVA diagnostic protocol field strain Lumpy skin disease virus Real-time PCR vaccine strain. ...
Retrospective survey of unauthorized genetically modified bacteria harbouring antimicrobial resistance genes in feed additive vitamin B2 commercialized in Belgium: Challenges and solutions
feed additive vitamin B2 products. To this end, two real-time PCR markers specific to this unauthorized genetically modified microorganism (GMM), named UGMV it- B2 and 558, were used. In the present ...
Challenges to Differentiate Hepatitis C Genotype 1 and 6: Results from A Field-Study in Cambodia.
LightPower Taqman real-time PCR method (Viet A Corp.) and quality controlled using the Versant 2.0 line probe assay (Siemens Healthcare). Next, following interim quality control results, all 106 samples were ...
The lack of K13-propeller mutations associated with artemisinin resistance in Plasmodium falciparum in Democratic Republic of Congo (DRC).
then confirmed by a P. falciparum real-time PCR assay. A pfk13-propeller segment containing a fragment that codes for amino acids at positions 427-595 was amplified by conventional PCR before sequencing. ...
Development of a genoserotyping system for the identification of Salmonella serotypes
Publication Type: Dissertation Authors: Mathieu Gand Source: Ghent University, Ghent, p.185 (2020) Keywords: Salmonella; Genoserotyping; Luminex; Decision support system; Food safety; real-time pcr ...
Molecular surveillance of anti-malarial drug resistance in Democratic Republic of Congo: high variability of chloroquinoresistance and lack of amodiaquinoresistance.
detected by a rapid diagnostic test (RDT) available on site (SD Bioline Malaria Ag Pf or CareStart Malaria Pf) or thick blood smear and then confirmed by a P. falciparum species-specific real-time PCR assay. ...
Selection of a Noninvasive Source of Human DNA Envisaging Genotyping Assays in Epidemiological Studies: Urine or Saliva?
Biomolecular Techniques: JBT, Volume 31, Issue 1 (2020) Keywords: Biomarkers children. genetic epidemiology Genotype Real-time PCR Abstract: Genetic epidemiology requires an appropriate approach to measure ...