Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking

Last updated on 6-12-2022 by Andrea Gobbo

Peer reviewed scientific article




A PCR-based DNA walking analysis was performed on a protease product suspected to contain a new unauthorized genetically modified microorganism (GMM). Though the characterization of unnatural associations of sequences between the pUB110 shuttle vector and a Bacillus amyloliquefaciens gene coding for a protease, the presence of the GMM was shown. Based on these sequences of interest, a real-time PCR marker was developed to target specifically the newly discovered GMM, namely GMM protease2. The performance of the real-time PCR marker was assessed in terms of spec…

Associated health topics:

QR code

QR code for this page URL