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Occurrence and diversity of free-living protozoa on butterhead lettuce.

Identification of FLP was performed by light microscopy and sequencing of denaturing gradient gel electrophoresis (DGGE)-separated 18S rRNA gene fragments. Bodo saltans, Spumella (-like) spp. and Cercozoa were the ...

Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression.

(2010) Keywords: Animals Antibodies, Monoclonal Antigens, CD45 Blood Platelets Cell Separation Chickens Erythrocytes Flow Cytometry Interleukin-1beta Interleukin-6 Leukocytes Phycoerythrin Staining and ...

Microscopic and molecular studies of the diversity of free-living protozoa in meat-cutting plants.

sequencing of PCR-amplified, denaturing gradient gel electrophoresis (DGGE)-separated 18S rRNA gene fragments, which was used as a fast screening method. The general results of the survey showed that ...

Isolation and characterization of Helicobacter suis sp. nov. from pig stomachs.

Sequencing of the 16S rRNA gene, the 23S rRNA gene, the partial hsp60 gene and partial ureAB genes confirmed that the strains present in the gastric mucosa of pigs constituted a separate taxon, corresponding ...

Validation of a real-time PCR assay for the detection of bovine herpesvirus 1 in bovine semen.

separately to BoHV-1 PCR, or in a duplex format. The real-time PCR assay is intended for use in international trade. The complete validation dossier based on this study and an international inter-laboratory ...

Evidence of indirect transmission of classical swine fever virus through contacts with people.

inoculated and susceptible pigs were housed in separate compartments, between which the airborne transmission of the virus was impossible. A worst-case scenario with an intensive visiting protocol and no form ...

Development and application of analytical methods for the determination of mycotoxins in organic and conventional wheat.

neutral mycotoxins were separated by a linear gradient and detected by double-mode (ultraviolet light fluorescence) detection. The average DON, ZEN and OTA recovery rates from spiked blank wheat flour were ...

The diversity of Mycoplasma hyopneumoniae within and between herds using pulsed-field gel electrophoresis.

flocculare species clustered separately from all M. hyopneumoniae isolates, forming the root of the dendrograms. The PFGE patterns of the M. hyopneumoniae isolates of different herds were highly diverse and ...

Host- and tissue-specific pathogenic traits of Staphylococcus aureus.

in one of the five main clusters of the AFLP database (cluster I). In essence, mastitis-associated strains from animals were categorized separately (cluster IV a) and cosegregated with ...

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