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Microsatellite typing of Aspergillus fumigatus isolates recovered from deep organ samples of patients with invasive aspergillosis36813
Repeats Mycological Typing Techniques Organ Specificity Patient patients Print Research Research Support Sample Samples SB- IM Sites strain Universities university Abstract: Microsatellite typing was used ...
Comparative studies on the pathogenicity and tissue distribution of three virulence variants of classical swine fever virus, two field isolates and one vaccine strain, with special regard to immunohistochemical investigations.
elapsed post infection. Virus spread in various organs was followed by virus isolation, by immunohistochemistry, applying monoclonal antibodies in a two-step method and by in situ hybridisation using ...
Immunogenicity and protective efficacy of DNA vaccines encoding MAP0586c and MAP4308c of Mycobacterium avium subsp. paratuberculosis secretome.
mice with recombinant protein resulted in strong antibody responses against both proteins. Using synthetic overlapping peptides, immunodominant H-2(d) and H-2(b) restricted Th1 T cell epitopes were ...
Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology.
Publication Type: Peer reviewed scientific article Authors: Letellier, Carine; Boxus, Mathieu; Rosar, Laurent; Toussaint, Jean-François; Walravens, Karl; S. Roels; Meyer, Gilles; Letesson, Jean-Jacques; Pierre Kerkhofs Source: Vaccine, Volume 26, Issue 37 ...
Effect of pooling and multiplexing on the detection of bluetongue virus RNA by real-time RT-PCR.
(RT-qPCR) was used routinely for laboratory diagnosis during the 2006/2007 bluetongue virus (BTV) serotype 8 epidemic. In the present study the impact of pooling and multiplexing strategies on RT-qPCR are ...
Bluetongue virus serotype 8-associated congenital hydranencephaly in calves.
using a BTV-specific real-time RT- PCR with a much higher success rate in brain tissues compared with blood and spleen samples. Virus isolation in embryonated eggs was unsuccessful. In conclusion, ...
Microscopic and molecular studies of the diversity of free-living protozoa in meat-cutting plants.
sequencing of PCR-amplified, denaturing gradient gel electrophoresis (DGGE)-separated 18S rRNA gene fragments, which was used as a fast screening method. The general results of the survey showed that ...
A Bayesian evaluation of six diagnostic tests for foot-and-mouth disease for vaccinated and non-vaccinated cattle.
unknown, a Bayesian analysis employing a latent class model was used that did not rely on the use of a reference test or gold standard. Prior information about prevalence for subsets of the data and ...
Detection of Neospora caninum in dog organs using real time PCR systems23
can harbour enkysted parasites in several organs. The detection of N. caninum was performed using 3 different real time PCR systems all amplifying the NC5 DNA region. One system was based on Sybrgreen, ...
Indirect foot-and-mouth disease vaccine potency testing based on a serological alternative.
and different serological assays were used, including virus neutralisation assays and ELISA formats. Models correlating specific anti- FMD virus antibody titres to protection were built using logistic ...