Detection of hepatitis D virus by qPCR

Please fill in entirely the request form from the NRC for all analysis requests. You can download it below.

 

Pathogen

Hepatitis D Virus (HDV)

Assay description

LOINC Codes :  

• 7906-1
• 85512-2
• 85513-0

Details : 

Assay perfomed with a commercial PCR kit : RealStar HDV RT-PCR 1.0 (Altona Diagnostics).

Results : 

Quantitative (IU/mL and log IU/mL).

The result of the quantification is only reported if the viral load is comprised between the limits of quantification defined by the standard curve (429 IU/mL – 429 000 IU/mL).

If the viral load is lower than the lower limit of quantification or above the upper limit of quantification that are defined by the standard curve, the result of the quantification will be given for information purpose only.

The limit of detection of our assay is of 13.4 IU/mL.

Accreditation : 

Accredited assay.

ISO 15189 | Quality and competence in medical laboratories

Aim of the assay 

HDV RNA detection by real time quantitative PCR.

Diagnosis of a HDV infection.

Criteria 

The patient must be positive for HDV serology and/or Hepatitis B Virus surface antigen (HBs Ag) and/or Hepatitis B Virus DNA (HBV PCR). The requesting lab must mention the clinical details suggestive of an infectious hepatitis on the request form.

HDV PCR is automatically performed in case of positive/undetermined HDV-Ig_Tot performed in our laboratory. Reporting is done once the analysis is finished.

Instructions related to sample

Type : Serum

Conservatory and shipment conditions : 

Conservation at 4°C before shipment.

Shipment at ambient temperature as soon as possible in a hermetic three layer package.

Requested quantities :

Preferred : 1 ml.

Minimal requirements : 0,5 ml.

Rejected request

Sample with insufficient volume.

No positive results for HDV serology and/or HBs Ag and/or HBV PCR performed prior to the request and for which results are not mentioned on the request form.

Turnaround time

TAT : 7 opening days

Reporting

An analysis report can be sent by regular mail, e-mail, fax ou eHealth Box for each analysis request.