Last updated on 17-12-2024 by Amber Van Laer
Description of the test
Whole-genome sequencing (WGS) is performed on STEC isolates to complement the results of systematic typing.
WGS-based typing:
- O:H-type
- Other variants of the stx gene (stx1a, c, d and stx2b, c, e, g)
- aaiC and aggR: enteroaggregative genes
- ehxA: enterohemolysin
- core genome multilocus sequence typing (cgMLST) sequence type (ST)
Purpose of the test
Contribution to study the epidemiology of STEC infections.
Criteria for performing this test in the context of reference activities
- STEC strains positive for stx2 with the exception of stx2f
- STEC strains from HUS cases
- STEC strains from outbreaks
- STEC strains belonging to serotypes O26 and O157
Note: WGS can be performed on request for criteria other than those listed above. Please contact the NRC for this purpose.
Instructions for samples
Not applicable
Instructions for transport
Not applicable
Unacceptable requests
Not applicable
Turn around time (and frequency of analysis)
1 month
Note: The frequency of implementation depends on the epidemiological situation.
Reporting of test results
- The cgMLST ST is reported via mail
- Global reporting of additional test results in annual report
- Interim results are sent to the ECDC (via Sciensano)
Accreditation
Is the analysis accredited?
Associated certificate(s):
Materials and methods
Material(s):
Method reference:
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Request forms
URL to form(s):
Analysis categories
Medical