Description of the test
- DNA extraction via NucliSens easyMAG/eMAG (BioMérieux)
- MucorGenius (Pathonostics) analysis via LightCycler 480II (Roche) (real-time PCR and melting curves)
Purpose of the test
Detection of Mucorales species DNA (Rhizopus species, Mucor species, Lichtheimia species, Cunninghamella species and Rhizomucor species) — diagnosis of mucormycosis
Criteria for performing this test in the context of reference activities
Suspicion of a fungal infection in a patient at risk for fungal infections where a normal sterile sample (biopsy or puncture fluid) is collected.
Presence of fungal threads in the direct microscopic examination of a normal sterile sample.
Patient with possible invasive mycosis (based on host factors and radiology) in which the galactomannan determination and culture of the BAL fluid is negative (an additional request on the BAL fluid is possible in that context).
Instructions for samples
- Sample: biopsy, bronchoalveolar lavage (BAL), serum (plasma) or normal sterile body fluid (various fluids)
- Storage at 2-8°C
- Longer than 10 days: freeze at -20°C / -70°C
Instructions for transportation
Transport at room temperature if less than 2 days, otherwise refrigerated transport
Unacceptable requests
Application form not fully completed, criteria not met
Turn around time (and frequency of analysis)
- 1 working day
- Output frequency: every working day, during duty hours (batch)
Reporting of test results
Paper report, email or fax as preferred by client.
Financing
Billing (€53)