For the serological diagnosis of cystic echinococcosis, a combination of an anti-E. granulosus IgG chemiluminescent immunoassay (CLIA) (sensitive test) followed by confirmation of specificity by a western blot (highly sensitive and specific test) is used. The antigens used for this ELISA test are hydatid fluid antigens.
- IgG anti-E. granulosus CLIA test
This test is performed whenever cystic echinococcosis is suspected.
- Western blot
This test is used if the CLIA IgG anti-E. granulosus test is positive, to confirm specificity. When this test is negative despite equivocal clinical and epidemiological evidence of echinococcosis, the western blot is still used as a screening method to confirm the negative result. The NRL’s experience of screening for cystic echinococcosis has demonstrated the usefulness of this technique, which, in addition to its high specificity, can also detect false negatives obtained by enzyme immunoassay methods.
Instructions regarding the sample
- Sample type: serum
- Conditions for storage: conservation at 2-8°C
- Required quantity: minimum 3-5 mL
Instructions for transportation
- It is recommended that the serum be transported at a temperature of between 2 and 8°C.
- Labeling and conditions complying with the rules concerning infectious samples, containers, and packaging are applicable.
Demandes that cannot be accepted
If the request form is missing or if it’s incomplete.
Timeframe for receiving test results (and frequency of analysis)
- Tests are carried out once a week. Results are available the same day or a week later if control is required.
- The “turnaround time” (TAT) for this type of analysis is 7 days.
Communication of the results
The results are communicated via a report sent by post or electronically. Positive results are communicated by telephone and faxed to the clinician.