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Detection and Molecular Characterization of Foot and Mouth Disease Viruses from Outbreaks in Some States of Northern Nigeria 2013-2015.
FMD-affected animals using rRT- PCR, and cytopathic effect was found in cell culture by 59% (44 of 74) of these samples. Three FMDV serotypes O, A and SAT2 were isolated and characterized. The phylogenetic ...
Endocrine activity of mycotoxins and mycotoxin mixtures.
Source: Food Chem Toxicol, Volume 96, p.107-16 (2016) Keywords: Breast Neoplasms Cells, Cultured Endocrine disruptors Female Genes, Reporter Humans Luciferases Mycotoxins Osteoblasts Poisons PPAR gamma ...
Pseudorabies virus isolates from domestic pigs and wild boars show no apparent in vitro differences in replication kinetics and sensitivity to interferon-induced antiviral status.
Animals Antiviral Agents Cells, Cultured Herpesvirus 1, Suid Interferons Sus scrofa Viral Plaque Assay Virus Replication Abstract: Pseudorabies virus is the causative agent of Aujeszky’s disease. Domestic ...
Next-generation sequencing shows West Nile virus quasispecies diversification after a single passage in a carrion crow (Corvus corone) in vivo infection model.
diversity estimates in sampled wild birds and mosquitoes, and in multiple-passage adaptation studies in vivo or in cell culture, suggest that WNV genetic diversification is mostly limited to the mosquito ...
Comparison of sample types and analytical methods for the detection of highly campylobacter-colonized broiler flocks at different stages in the poultry meat production chain.
samples. PMA treatment before qPCR inhibits the signal from dead Campylobacter cells. Consequently, when samples are extensively stored and/or transported, qPCR is preferred to direct culture and PMA-qPCR. ...
Pre-registration efficacy study of a novel marker vaccine against classical swine fever on Maternally Derived Antibody negative (MDA-) target animals.
prepared in cell cultures), classical” 7th Edition, which claims the efficacy test is invalid if fewer than 50 per cent of the control piglets display typical signs of serious infection of CSF or die, and if ...
In vitro surrogate models to aid in the development of antivirals for the containment of foot-and-mouth disease outbreaks.
cell culture system has been reported so far for BRBV, we demonstrate that infection of primary bovine kidney cells resulted in an extensive but rather poorly-reproducible induction of cytopathic effect ...
Interaction of Aspergillus fumigatus conidia with Acanthamoeba castellanii parallels macrophage-fungus interactions.
common inhabitants of soil. Mechanisms of A. fumigatus to circumvent the amoeba’s digestion may facilitate overcoming the vertebrate macrophage defence mechanisms. We performed co-culture experiments using ...
Characterization of methicillin-resistant Staphylococcus aureus from healthy carrier chickens.
p.342-6 (2013) Keywords: Animals Belgium Cell Culture Techniques Chickens Drug Resistance, Bacterial Erythromycin Likelihood Functions Methicillin-Resistant Staphylococcus aureus Multilocus Sequence Typing ...
Impact of a novel inactivated PRRS virus vaccine on virus replication and virus-induced pathology in fetal implantation sites and fetuses upon challenge.
tissue culture infectious dose 50 (TCID (50)) of PRRSV 07V63. Twenty days postchallenge animals were euthanized and sampled. The vaccinated gilts quickly developed virus neutralizing (VN) antibodies ...