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Prioritisation of food-borne parasites in Europe, 2016.

following a recommendation from the Food and Agriculture Organization of the United Nations (FAO) and World Health Organization (WHO), 35 European parasitologists attended a workshop from 8-12 February 2016 ...

Comparison of Droplet Digital PCR and qPCR for the Quantification of Shiga Toxin-Producing Escherichia coli in Bovine Feces.

genes stx1 and stx2 and the intimin gene eae quantification was optimized for ddPCR analysis. The properties of ddPCR and qPCR using two different mastermixes (EMM: TaqMan(®) Environmental Master Mix 2.0; ...

RNA sequencing-based transcriptional overview of xerotolerance in Cronobacter sakazakii SP291.

Infant Formula RNA, Bacterial Sequence Analysis, RNA Transcription, Genetic Trehalose Abstract: Cronobacter sakazakii  is a xerotolerant neonatal pathogen epidemiologically linked to powdered infant food ...

Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates.

Nextera XT library preparation; (ii) MiSeq sequencing (data quality, read mapping against plasmid and chromosome references); and (iii) WGS data analysis, i.e. isolate characterization (serotyping, ...

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