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Detailed Evaluation of Data Analysis Tools for Subtyping of Bacterial Isolates Based on Whole Genome Sequencing: as a Proof of Concept.

Volume 10 (2019) Abstract: Whole genome sequencing is increasingly recognized as the most informative approach for characterization of bacterial isolates. Success of the routine use of this technology in ...

Development of a real-time PCR method for the genoserotyping of Salmonella Paratyphi B variant Java.

Bacteriële ziekten Bacterial diseases Activités transversales en génomique appliquée Transversale activiteiten in toegepaste genomica Transversal activities in applied genomics Manuscript versions:  DOI:  ...

Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.

responsible for millions of episodes of bacterial dysenteriae worldwide. Given its distinct epidemiology and public health relevance, only Shigellae are subject to mandatory reporting and follow-up by public ...

Occurrence and identification of spore-forming bacteria in skim-milk powders

bacterial and spore enumeration tests to understand the variety of spore-forming bacteria population. Using 16S rDNA sequencing, the species were identified for 269 isolates that were representative of the ...

Prediction of Susceptibility to First-Line Tuberculosis Drugs by DNA Sequencing

Publication Type: Peer reviewed scientific article Authors: The CRyPTIC Consortium and the 100000 Genomes Project; Allix-Béguec, Caroline; Irena Arandjelovic; Bi, Lijun; Patrick Beckert; Maryline ...

Preparing cDNA Libraries from Lytic Phage-Infected Cells for Whole Transcriptome Analysis by RNA-Seq.

Bacteriophage RNA-seq Abstract: Whole genome wide analysis of transcription using RNA-Seq methods is a powerful way to elucidate differential expression of gene features in bacteria across different conditions as ...

Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:.

Salmonella; Salmonella enterica; Salmonella typhimurium; SNP; subtyping; whole genome sequencing Abstract: Whole genome sequencing represents a promising new technology for subtyping of bacterial pathogens. ...

A cluster of multidrug-resistant Mycobacterium tuberculosis among patients arriving in Europe from the Horn of Africa: a molecular epidemiological study.

profiles; phenotypic resistance to isoniazid, rifampicin, ethambutol, pyrazinamide, and capreomycin; and corresponding drug resistance mutations. We whole-genome sequenced all Mycobacterium tuberculosis ...

Virulence and immunogenicity of genetically defined human and porcine isolates of M. avium subsp. hominissuis in an experimental mouse infection

compared the virulence and immunogenicity of porcine and clinical human isolates with shared genotype or with a genotype only found in humans or pigs. Bacterial replication was monitored for 20 weeks in ...

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