Search results - 174 results
Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.
RT-qPCR failure due to poor sample quality, improper nucleic acid extraction and/or PCR inhibition. To increase the diagnostic capacity and reduce costs, it is recommended to use a multiplex strategy which ...
The positive adjuvant effect of chitosan on antigen-specific cell-mediated immunity after chickens vaccination with live Newcastle disease vaccine.
It has been demonstrated to be an effective absorption enhancer to improve mucosal delivery of peptide and protein drugs in human and mice. In poultry, mucosal administration of live vaccine has been ...
Passive protection afforded by maternally-derived antibodies in chickens and the antibodies' interference with the protection elicited by avian influenza-inactivated vaccines in progeny.
(HPAIV), and its suspected interference with current inactivated vaccines in broilers under controlled laboratory conditions. In the first set of experiments, broilers were vaccinated with inactivated ...
Fine quantification of avian influenza H5N1 escape mutant quasispecies populations using mutation-specific real-time PCR.
approach allows in-depth analysis of quasispecies dynamics using large sample sizes. It may also be applied to the dynamics of hot spots of mutations in several genes, such as HA or PB2, and to the early ...
Identification of a dominant epitope in the hemagglutinin of an Asian highly pathogenic avian influenza H5N1 clade 1 virus by selection of escape mutants.
humans. The hemagglutinin (HA) is a glycoprotein on the surface of the virus envelope. Understanding its antigenic structure is essential for designing novel vaccines that can inhibit virus infection. The ...
Rapid detection of Eurasian and American H7 subtype influenza A viruses using a single TaqManMGB real-time RT-PCR.
determined with the use of serial dilutions of Eurasian isolates A/Ck/ BE /06775/2003 and A/Ck/It/1067/v99 and North American isolates A/ CK / PA / 143586/2001 and A/Quail/ PA /20304/1998, to be 1 log10 higher ...
Evaluation of different serologic markers for the early detection of avian influenza infection in chickens.
influenza viruses can mutate during circulation in poultry and emerge as high pathogenicity avian influenza variants. It is therefore essential to monitor the field situation continuously to detect low ...
H5N1 high pathogenicity avian influenza virus survival in different types of water.
twice a week over a 60-day trial period by microtiter endpoint titration. The persistence of the virus varied considerably depending on its concentration and also on physico-chemical parameters of the ...
Transfer of soil contaminants to home-produced eggs and preventive measures to reduce contamination.
produced eggs. It was the aim of this study to identify the major source of two environmental pollutants in home-produced eggs, i.e. dioxins and lead, to formulate preventive measures to reduce the ...
Quantification of foot-and-mouth disease virus transmission rates using published data.
sheep to in-contact pigs. This new analysis utilises a state-of-the-art Generalised Linear Model to estimate the transmission rate. From the obtained results it is concluded that meta-analysis of “old” ...