Search results - 9 results

A more sensitive, efficient and ISO 17025 validated Magnetic Capture real time PCR method for the detection of archetypal Toxoplasma gondii strains in meat.

presented results demonstrate the potential of the modified Magnetic Capture real time PCR as a full alternative to the mouse bioassay for the screening of various types of tissues and meat, with the ...

Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study.

(PCR) and the real-time PCR are the most frequently used tools for indoor fungal detection. However, even if these tools have demonstrated their appropriate performance, some of them are not able to ...

Erratum to: Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.

Publication Type: Peer reviewed scientific article Authors: Libert, X; Camille Chasseur; Bladt, S; Ann Packeu; Bureau, F; Nancy Roosens; Sigrid C.J. De Keersmaecker Source: Appl Microbiol Biotechnol, Volume 99, Issue 19, p.8319-20 (2015) Health Topics:  F ...

Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.

excellent alternative for the monitoring of indoor fungal contaminations. As a case study, we developed a SYBR ® green real-time PCR-based assay for the specific detection and identification of Aspergillus ...

Statistical framework for detection of Genetically Modified Organisms based on Next Generation Sequencing

number and diversity of genetically modified (GM) crops has significantly increased, their analysis based on real-time PCR (qPCR) methods is becoming increasingly complex and laborious. While several ...

New trait-specific qualitative SYBR®Green qPCR methods to expand the panel of GMO screening methods used in the CoSYPS

diverse. Consequently, the screening approaches have to be updated to enable full coverage and better discrimination of all these events. To respond to this need, two new qualitative SYBR ®Green real-time ...

Comparative study of seven commercial kits for human DNA extraction from urine samples suitable for DNA biomarker-based public health studies.

species-specific real-time quantitative (q) PCR assays, as DNA extracted from urine contains, besides human, microbial DNA also, which largely contributes to the total DNA yield. In addition, the kits giving a good ...

Evaluation of viability-qPCR detection system on viable and dead Salmonella serovar Enteritidis.

103, p.131-7 (2014) Keywords: Azides Microbial Viability Propidium Real-Time Polymerase Chain Reaction Salmonella enteritidis Salmonella Infections Sensitivity and Specificity Abstract: The propidium ...

A two-step lyssavirus real-time polymerase chain reaction using degenerate primers with superior sensitivity to the fluorescent antigen test.

two-step lyssavirus real-time reverse transcriptase polymerase chain reaction (qRT- PCR), based on a nested PCR strategy, was validated for the detection of different lyssavirus species. Primers with 17 to ...

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