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Comparison of Serological Methods for Tick-Borne Encephalitis Virus-Specific Antibody Detection in Wild Boar and Sheep: Impact of the Screening Approach on the Estimated Seroprevalence.
(TBEV) IgG All Species (Progen) and ID Screen West Nile Competition (Innovative Diagnostics)) and PRNT s for TBEV and USUTU virus. The results showed that the Immunozym and IDS creen ELISA s had low ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
level, were specifically designed for this application and need to be assessed. Results: In this study, with different bacterial cocktail mixes, analyzed as pure or spiked in a synthetic fecal matrix, we ...
Development of a Taxon-Specific Real-Time Polymerase Chain Reaction Method to Detect Trichoderma reesei Contaminations in Fermentation Products
food industry to produce fermentation products, a novel real-time PCR method specific to this fungal species was developed and validated in this study to reinforce the GMM detection strategy. Designed to ...
Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms
analytical and interpretation levels since this requires methodological approaches that can target and detect a specific single nucleotide variation (SNV) introduced into a GE organism. In this study, we ...
MALDI-TOF Mass Spectrometry Online Identification of Using the MSI-2 Application.
spread from India to Europe and that is more prone than other species of the complex to show resistance to terbinafine, resulting in the necessity of rapid identification. Here, we improved the online ...
Taenia solium Cysticercosis/Taeniosis in Europe and Central Asia
in reviews, taeniosis and cysticercosis cases were reported in almost all countries across the continent. Species specification was rarely performed and diagnosis of human cysticercosis was challenging ...
Development of a taxon-specific real-time PCR method targeting the Bacillus subtilis group to strengthen the control of genetically modified bacteria in fermentation products
contaminations consisted of GM Bacillus species belonging to the B. subtilis group. In order to screen for the potential presence of such contaminations, in this study we have developed a new real-time PCR ...
Shotgun metagenomics as a ONE Health tool for better protecting human health
species in various concentrations and carrying AMR and/or virulence genes, taking the specific need of each application into account. Moreover, the short read and long read approaches are being compared to ...
Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida (Coleoptera: Nitidulidae): A European Inter-Laboratory Comparison.
species. To ensure the reliability of official diagnoses in case of introduction, an inter-laboratory comparison was organised on the identification of by morphology and real-time PCR. Twenty-two National ...
Retrospective evaluation of routine whole genome sequencing of Mycobacterium tuberculosis at the Belgian National Reference Center, 2019.
bioinformatics workflow to determine the species and antimicrobial resistance. In this study, we retrospectively compare results obtained via WGS to conventional phenotypic and genotypic testing, for all Belgian ...