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Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study.

sensitivity and specificity. CONCLUSIONS: We provide a comprehensive overview of dengue virus RT-qPCR method performance that will aid appropriate method selection allowing to take specific measures that aim to ...

Relationship between mold exposure, specific IgE sensitization, and clinical asthma: A case-control study.

alternata, Cladosporium herbarum, and Aspergillus fumigatus. However, species such as Penicillium spp, Cladosporium sphaerospermum, Cladosporium cladosporioides, or Aspergillus versicolor display a more ...

Tobacco, Sunflower and High Biomass SRC Clones Show Potential for Trace Metal Phytoextraction on a Moderately Contaminated Field Site in Belgium.

valorization. To find out which high biomass crop possessed the highest and most constant (in time) phytoextraction potential on these soils, different plant species and different mutants or clones of each ...

New insights in dermatophyte research.

such as Sub6 may have emerged as a result of ancestral gene duplication, and are likely to have specific roles during infection. Virulence may differ between mating partners of the same species and ...

A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods

a multi-laboratory validation study with the aim of improving the precision of this ISO technical specification and providing a single ISO standard method to enable detection of these important foodborne Vibrio spp.. ...

Brucella abortus is Prevalent in Both Humans and Animals in Bangladesh.

human sera and all animal samples were screened by Brucella genus-specific real-time PCR (RT- PCR), and positive samples were then tested by IS711 RT- PCR to detect B. abortus and B. melitensis DNA. Only ...

Comparative Tick-Borne Encephalitis (Virus) Surveillance in Belgium 2009-2015: Experiences with Diagnostic Tests, Sentinel Species and Surveillance Designs

Volume 1, Issue 4 (2017) Keywords: Tick-borne encephalitis (virus); Wild boar; Roe deer; Sentinel species; Abstract: When it is not overtly affecting human beings, the Tick-Borne Encephalitis Flavivirus ...

Fusarium, clinical relevance and mass spectrometry methods for identification, using strains of the Belgian fungal culture collection BCCM/IHEM

development of a new identification method for mold infections which is directly applicable on clinical samples and relies upon the detection of species- and genus-specific peptides using tandem mass ...

Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination.

study a Luminex xMAP® assay was developed for the simultaneous detection of 10 fungal species which are most frequently in indoor air and that may cause health problems. This xMAP® assay was found to be ...

Belgian Wildlife as Potential Zoonotic Reservoir of Hepatitis E Virus.

ELISA screening prevalence, Western blot (WB) analyses were carried out, a receiver operating characteristic curve analysis was performed and different scenarios with varying ELISA specificities relative ...

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