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Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of molds of the Fusarium genus.

Source: J Clin Microbiol, Volume 53, Issue 2, Number 476, p.465-76 (2015) Keywords: Belgium Fusariosis Fusarium Humans Microbiological Techniques Sensitivity and Specificity Spectrometry, Mass, ...

DNA vaccines against tuberculosis.

increase their immunogenicity in large animal species. DNA vaccines are able to induce a wide variety of immune responses, including CD8 (+) T-cell-mediated cytolytic and IFN-γ responses. DNA vaccination may ...

Are 20 human papillomavirus types causing cervical cancer?

Therefore, these types were considered as 1A carcinogens. They all belong to the family of the α-Papillomaviridae, in particular to the species α5 (HPV51), α6 (HPV56), α7 (HPV18, HPV39, HPV45, HPV59) and α9 ...

Comparative study of seven commercial kits for human DNA extraction from urine samples suitable for DNA biomarker-based public health studies.

species-specific real-time quantitative (q) PCR assays, as DNA extracted from urine contains, besides human, microbial DNA also, which largely contributes to the total DNA yield. In addition, the kits giving a good ...

A two-step lyssavirus real-time polymerase chain reaction using degenerate primers with superior sensitivity to the fluorescent antigen test.

with the remaining lyssavirus species. The diagnostic specificity was 100% and the sensitivity proved to be superior to that of the fluorescent antigen test. The limit of detection was ≤ 1 50% tissue ...

Comparison of different serological methods to detect antibodies specific to Neospora caninum in bovine and canine sera.564

kappa index were calculated for each host species and assay. For dog sera, the sensitivity and the specificity of the LAT was 76% and 100%, respectively. The McNemar test showed that the LAT was not ...

SYBR®Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels.

Sensitivity and Specificity Staining and Labeling Abstract: In this work, a three-level Salmonella detection system based on a combination of seven SYBR ®Green qPCR was developed. This detection system ...

Microbial characterization of probiotics--advisory report of the Working Group "8651 Probiotics" of the Belgian Superior Health Council (SHC)36501

This advisory report covers three main steps in the microbial quality assessment process, i.e. (i) correct species identification and strain-specific typing of bacterial and yeast strains used in ...

Experimental co-infections of calves with bluetongue virus serotypes 1 and 8.

a standardized clinical form. Serotype-specific real-time RT- PCR s and viral isolation were used to monitor the course of viraemia. Neutralizing antibody titres were measured during the experiments, and necropsy ...

M.tuberculosis mutants lacking oxygenated mycolates show increased immunogenicity and protective efficacy as compared to M. bovis BCG vaccine in an experimental mouse model.

Mutation Mycobacterium bovis Mycobacterium tuberculosis Mycolic Acids Organ Specificity Oxygen Species Specificity Spleen Th1 Cells Th17 Cells Trachea Treatment Outcome Tuberculosis Vaccination virulence ...

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