Search results - 49 results
Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling
analysing the DNA of the sample, it can identify the presence of various pathogens to the strain level. Moreover, adaptive sampling is a new tool proposed during nanopore sequencing to target certain DNA ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
genome copies). Finally, a targeting of the norovirus by hybridization capture enhanced the relative quantity of reads classified as norovirus but at the expense of using a less open approach that can only ...
Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms
analytical and interpretation levels since this requires methodological approaches that can target and detect a specific single nucleotide variation (SNV) introduced into a GE organism. In this study, we ...
Development of a reliable UHPLC-MS/MS method for simultaneous determination of zearalenone and zearalenone-14-glucoside in various feed products
impurities and enriching the target analytes in different feeds. The critical parameters affecting the performance of the solid-phase extraction (SPE) procedure were carefully optimized, and 20% acetonitrile ...
Development of a taxon-specific real-time PCR method targeting the Bacillus subtilis group to strengthen the control of genetically modified bacteria in fermentation products
method targeting the B. subtilis group, including B. subtilis, B. licheniformis, B. amyloliquefaciens and B. velezensis. The method’s performance was successfully assessed as specific and sensitive, ...
Development, Validation and Application of a Targeted LC-MS Method for Quantification of Microcystins and Nodularin: Towards a Better Characterization of Drinking Water
Publication Type: Peer reviewed scientific article Authors: Wannes Van Hassel; Bart Huybrechts; Julien Masquelier; Annick Wilmotte; Mirjana Andjelkovic Source: Water, Volume 14, Issue 8 (2022) Keywords: liquid chromatography Mass Spectrometry matrix effec ...
Sensitivity of candling as routine method for the detection and recovery of ascaridoids in commercial fish fillets.
make targeted recommendations to the fish industry, the Se was calculated per fish part, larval genus, and fish species. All fish parts (n = 615) were first candled, and larvae were collected, followed ...
Combined carbon and health taxes outperform single-purpose information or fiscal measures in designing sustainable food policies
exerts the most substantial effects on food purchasing decisions. Furthermore, while carbon or health taxes are best at separately targeting emissions or dietary health challenges, respectively, a combined ...
A Targeted UHPLC-MS/MS Method Validated for the Quantification of Ergot Alkaloids in Cereal-Based Baby Food from the Belgian Market
Publication Type: Peer reviewed scientific article Authors: Bart Huybrechts; Svetlana V. Malysheva; Julien Masquelier Source: Toxins, Volume 13, Issue 8 (2021) Keywords: baby food ergot alkaloids QuEChERS UHPLC-MS/MS VALIDATION Abstract: Following pending ...
Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking
developed to target specifically the newly discovered GMM, namely GMM protease2. The performance of the real-time PCR marker was assessed in terms of specificity and sensitivity. The applicability of the ...