Search results - 59 results
Development of a taxon-specific real-time PCR method targeting the Bacillus subtilis group to strengthen the control of genetically modified bacteria in fermentation products
and associated DNA) that were carrying antimicrobial resistance (AMR) genes was noticed in several microbial fermentation products that have been commercialized on the food and feed market. These ...
Shotgun metagenomics as a ONE Health tool for better protecting human health
composing genes such as virulence or antimicrobial resistance (AMR) genes, without prior knowledge, nor the need to isolate. Such approaches could offer efficient and fast solutions to limitations faced by ...
Nursing home residents with suspected urinary tract infections: a diagnostic accuracy study
The combined nitrite and leucocyte esterase urine dipstick test (one or both positive) had a 96.0% negative predictive value (95% CI: 80.5-99.3%). The sensitivity of a positive CRP test (≥ 5 mg/L) was ...
Retrospective evaluation of routine whole genome sequencing of Mycobacterium tuberculosis at the Belgian National Reference Center, 2019.
bioinformatics workflow to determine the species and antimicrobial resistance. In this study, we retrospectively compare results obtained via WGS to conventional phenotypic and genotypic testing, for all Belgian ...
Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking
Microbiology, Volume 354 (2021) Keywords: Antimicrobial resistance genes DNA walking anchored on pUB110 food and feed safety Genetically modified bacteria Protease real-time PCR detection Abstract: A PCR-based ...
A molecular assay for rapidly distinguishing the AviPro SALMONELLA VAC T vaccine strain from wild-type field isolates
wild-type Salmonella ser. Typhimurium isolates is essential for the monitoring of veterinary isolates and targeted control actions. The distinction between the two strain types is routinely made by phenotypic antimicrobial ...
A Bioinformatics Whole-Genome Sequencing Workflow for Clinical Mycobacterium tuberculosis Complex Isolate Analysis, Validated Using a Reference Collection Extensively Characterized with Conventional Methods and In Silico Approaches
Keersmaecker; Nancy Roosens; Marchal, Kathleen; Vanessa Mathys; Kevin Vanneste Source: J Clin Microbiol (2021) Keywords: Antimicrobial resistance Mycobacterium tuberculosis national reference center public health ...
Validation strategy of a bioinformatics whole genome sequencing workflow for Shiga toxin-producing Escherichia coli using a reference collection extensively characterized with conventional methods
performs complete characterization of Shiga toxin-producing Escherichia coli (STEC) isolates including antimicrobial resistance prediction, virulence gene detection, serotype prediction, plasmid replicon ...
Fluoroquinolone resistance in Escherichia coli isolates after exposure to non-fluoroquinolone antibiotics: a retrospective case–control study
Healthcare-associated infections and antimicrobial resistance Manuscript versions: DOI: https://doi.org/10.1093/jac/dkaa128 File: Version: Published Full text access: Restricted Access Full text language: English ...
Deep amplicon sequencing for culture-free prediction of susceptibility or resistance to 13 anti-tuberculous drugs.
isolates, the assay predicted 92.2% of 2369 first- and second-line phenotypes, with a sensitivity of 95.3% and specificity of 97.4%. Fifty-six of 69 (81.2%) residual discrepancies with phenotypic results ...