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Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling
Staphylococcus aureus) compared to shotgun metagenomics without adaptive sampling on DNA of mashed potatoes spiked with DNA of S. aureus at a level of 0.5%. The strain was previously associated with a foodborne ...
EFSA Project on the use of New Approach Methodologies (NAMs) for the hazard assessment of nanofibres. Lot 1, nanocellulose oral exposure: gastrointestinal digestion, nanofibres uptake and local effects
(iii) assessment of any digestion or degradation of NC by the human microbiome. Eight NC samples belonging to the three NC types, plus a comparator in the micro-range, were selected as study materials and ...
Shotgun metagenomics as a ONE Health tool for better protecting human health
microbiology. Compared to short read sequencing (Illumina), this technology allows to unambiguously detect and scaffold microbial genes to their host chromosomes, even for complex metagenomics samples, allowing ...
Migration of mineral oil from cardboard packaging for food: Identification of the hazards and assessment of the exposure of the Belgian population
The detected MOSH and MOAH concentrations were then compared to the action thresholds set by the Scientific Committee (SciCom) of the Belgian Food Safety Agency (FASFC) in 2017. Only one sample exceeded ...
Biological activity conferred by endocrine disrupting chemicals in hospital effluent and river water from the Brussels Region, Belgium
assess endocrine activity by comparing the mixture or cocktail effect originating from the sample extract. Added value is that this technique picks up effects from compounds that are not ...
A proteomics approach for the quantification of Staphylococcus aureus enterotoxins a and b in several matrices using uplc-msms analysis.
validating this method according to ISO 2002/657/ EC. When comparing the MS spectra peaks found for the IS and the endogenous peptides we were able to identify and quantify the presence of SEA and/or SEB in ...
Design of multiplex assays for molecular subtyping of pathogens with the Luminex xMAP technology
and cost of experimentation.We have compared two commercial software packages for the development and in silico simulation of a ligation dependent amplification (LDA) assay and a direct hybridization ...
An Alternative Method Validation Strategy for the European Decision 2002/657/EC
performance characteristic is not increased since the degrees of freedom are equal or higher compared to the conventional validation plan. Only 24 samples (27 samples for a balanced design) are needed with this ...
Pyrrolizidine alkaloids in herbs and related foodstuffs (PASHERBS)
states: between the median and maximum for all age groups. Compared with the EFSA assessment for the Belgian FCS2004, we note a substantial increase in the exposure. It could be explained by different ...
Misreporting of energy intake in the belgian food consumption surveys (2004-2014)
compared with FCS2004 for participants aged 15-64. Results Overall in 2014, 24.3% of participants underreported, 75.2% were plausible reporter and 0.5% overreported. Females underreported more often than ...