Search results - 33 results
An interlaboratory proficiency test using metagenomic sequencing as a diagnostic tool for the detection of RNA viruses in swine fecal material.
types. The suitability of experimental design to a given pathogen/sample matrix combination, quality assurance, interpretation, and follow-up investigation remain critical factors for the diagnostic ...
Advances in monitoring-centered quantification of microcystins in fish matrix using UHPLC-MS/MS
a plethora of different fish species. While reporting quantified cyanotoxins in multiple fish species, most studies do not take into account the potential differences of matrix for quantification in fish ...
Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling
a proof-of-concept, we explored the use of adaptive sampling using various databases (depletion of the food matrix i.e. potato, enrichment of a selection of foodborne pathogens, enrichment of a database of ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
bivalve). These samples were not enriched as it is particularly arduous to culture these viruses in laboratory conditions. Because the contamination level was low in a complex matrix, we tested several sample ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
level, were specifically designed for this application and need to be assessed. Results: In this study, with different bacterial cocktail mixes, analyzed as pure or spiked in a synthetic fecal matrix, we ...
Development, Validation and Application of a Targeted LC-MS Method for Quantification of Microcystins and Nodularin: Towards a Better Characterization of Drinking Water
Keywords: liquid chromatography Mass Spectrometry matrix effect Method validation microcystin WATER Abstract: Water availability and quality are two crucial factors contributing to a healthy and ...
Development, validation and application of multi-class methods for the analysis of food additives by liquid chromatography coupled to tandem mass spectrometry
sweeteners, preservatives, and antioxidants in various foods (e.g. beverages, dairies, processed meals). The methods were also validated in terms of selectivity, linearity, matrix effect, limit of ...
Adjunct Culture of Non-Starter Lactic Acid Bacteria for the Production of Provola Dei Nebrodi PDO Cheese: In Vitro Screening and Pilot-Scale Cheese-Making.
were genetically identified by Rep- PCR genomic fingerprinting, using the (GTG)-primer, and by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI- TOF MS). Twenty-seven ...
Adjunct Culture of Non-Starter Lactic Acid Bacteria for the Production of Provola Dei Nebrodi PDO Cheese: In Vitro Screening and Pilot-Scale Cheese-Making
(GTG)5-primer, and by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI- TOF MS). Twenty-seven strains, included in the qualified presumption of safety (QPS) list, were tested for ...
Simultaneous determination of parabens, bisphenols and alkylphenols in human placenta by ultra-high performance liquid chromatography-tandem mass spectrometry
negative electrospray ionization. Labeled internal standards and matrix-matched calibration are used for quantification of the compounds. The method was validated according FDA guideline for bio analytical ...