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Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling
resolved. Shotgun metagenomics is a sequencing method involving the analysis of the genetic material directly extracted from a sample, without the need for an isolation of the bacteria. By sequencing and ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
Keywords: DNA extraction HMW DNA Long-read Metagenomics Method evaluation nanopore Abstract: Background: Oxford Nanopore Technologies (ONT) offers an accessible platform for long-read sequencing, which ...
Shotgun metagenomics as a ONE Health tool for better protecting human health
each other, and also to the results obtained with conventional methods. Results: A commercial kit was selected and optimized for the extraction of sufficient high-molecular weight DNA, to match the needs ...
Optimization and validation of an analytical method for the quantification of short- and medium-chained chlorinated paraffins in food by gas chromatography-mass spectrometry
ECNI / MS). A dispersive solid phase extraction (dSPE) method was optimized for fish, meat, oil, milk and whole-grain cereal followed by clean-up with concentrated sulfuric acid and acid silica. ...
Development and application of a non-target extraction method for the analysis of migrating compounds from plastic baby bottles by GC-MS.
contact food contact material food contact materials Foods GC-MS identification IS legislation Less Mass method Migration milk MS need Non-target observed ON organic Pa pattern PATTERNS perceived plastics ...
Investigation of the Polycyclic Aromatic Hydrocarbons contamination in spices and dried herbs available on the Belgian market
food category for the Belgian market. Hence, there was a need to validate a method to quantify these compounds and investigate to check the compliance of spices and dried herbs available on the ...