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Using a combination of short- and long-read sequencing to investigate the diversity in plasmid- and chromosomally encoded extended-spectrum beta-lactamases (ESBLs) in clinical Shigella and Salmonella isolates in Belgium

Publication Type: Peer reviewed scientific article Authors: Berbers, Bas; Vanneste, Kevin; Roosens, Nancy H. C. J.; Marchal, Kathleen; Pieter-Jan Ceyssens *; De Keersmaecker, Sigrid C. J. Source: Microbial Genomics, Volume 9, Issue 1 (2023) Health Topics: ...

An interlaboratory proficiency test using metagenomic sequencing as a diagnostic tool for the detection of RNA viruses in swine fecal material.

material and the extracted RNA. While different mNGS workflows for the generation of mNGS data were used in the different laboratories, the bioinformatic analysis was standardized using a metagenomic read ...

Benchmarking bacterial taxonomic classification using nanopore metagenomics data of several mock communities

sequencing. While second-generation Illumina sequencing still dominates, third-generation nanopore sequencing promises improved classification through longer reads. However, extensive benchmarking studies on ...

Metabarcoding and metagenomics techniques in monitoring airborne pollen

“GenapSys” technology and above all “Ilumina”, represent several platforms that sequence with the metabarcoding procedure short DNA fragments (reads) up to a few hundred base pairs in length. Other platforms ...

Towards facilitated interpretation of shotgun metagenomics long-read sequencing data analyzed with KMA for the detection of bacterial pathogens and their antimicrobial resistance genes

Publication Type: Peer reviewed scientific article Authors: Mathieu Gand; Navickaite, Indre; Bartsch, Lee-Julia; Grützke, Josephine; Overballe-Petersen, Søren; Rasmussen, Astrid; Otani, Saria; Michelacci, Valeria; Matamoros, Bosco Rodríguez; González-Zorn ...

Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens.

Whole-genome sequencing has become the method of choice for bacterial outbreak investigation, with most clinical and public health laboratories currently routinely using short-read Illumina sequencing. Recently, ...

Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens.

Whole-genome sequencing has become the method of choice for bacterial outbreak investigation, with most clinical and public health laboratories currently routinely using short-read Illumina sequencing. Recently, ...

Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens.

Whole-genome sequencing has become the method of choice for bacterial outbreak investigation, with most clinical and public health laboratories currently routinely using short-read Illumina sequencing. Recently, ...

Closing the gap: Oxford Nanopore Technologies R10 sequencing allows comparable results to Illumina sequencing for SNP-based outbreak investigation of bacterial pathogens.

with most clinical and public health laboratories currently routinely using short-read Illumina sequencing. Recently, long-read Oxford Nanopore Technologies (ONT) sequencing has gained prominence and may ...

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