Search results - 340 results
BSFM 2023- L. monocytogenes in Plant-Based Foods: insights from of a recent foodborne outbreak linked to vegan alternative to cheese
following ISO 11290 – 1 and – 2. A human strain was collected at the NRC Listeria. All isolated strains underwent serotyping for further characterisation at the NRC Listeria, and Whole Genome Sequencing (WGS) ...
Introduction of WGS in food microbiology: advantages and challenges
Keywords: antimicrobial resistance (AMR) Food Safety foodborne outbreak investigation Monitoring SciCom Whole genome sequencing (WGS) Abstract: In October 2021 the Scientific Committee of the Federal Agency ...
Listeria surveillance in Belgium
evolution in molecular typing, the NRC completely switched to Whole-Genome-Sequencing (WGS) for cluster detection. Therefore, an in-house Galaxy-based bio-informatic pipeline was developed. For the strain ...
Global transcriptional analyses of Mycobacterium tuberculosis using old and new drugs
constructed and sequenced using the HiSeq technology (Illumina®). Reads were mapped to the reference genome and normalized read counts were calculated per gene. Results: For each drug a specific ...
Global transcriptional analyses of Mycobacterium tuberculosis using old and new drugs
bead-beating in TRI zol and the Direct-Zol purification kit. After quality control, the extracts were fragmented, barcoded and pooled, cDNA libraries were constructed and sequenced using the HiSeq technology ...
Strategies to detect unauthorized genetically modified organisms in food and feed chain
routine analysis, consists to characterize sequences surrounding detected key transgenic elements using a DNA walking approach. By this way, the acquisition of sequences from the junction between the ...
Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices.
DNA walking methods, anchored on the sequences used for the p35S or tNOS qPCR screening, were tested on Bt rice that contains these two transgenic elements. Second, the methods were assessed on a maize ...
Statistical framework for detection of Genetically Modified Organisms based on Next Generation Sequencing
pioneers already investigated Next Generation Sequencing (NGS) as an alternative to qPCR, its practical use has not been assessed for routine analysis. In this study a statistical framework was developed to ...
Antimicrobial resistance and population structure of Staphylococcus epidermidis recovered from animals and humans.
Population Genotype Humans Livestock Methicillin Resistance Microbial Sensitivity Tests Multilocus Sequence Typing Staphylococcus epidermidis Abstract: While Staphylococcus epidermidis, as part of the ...
The usefulness of whole genome sequencing for outbreak investigation of food pathogens, Salmonella Enteritidis as a case study
genome whole genome sequencing Abstract: No abstract Service: Activités transversales en génomique appliquée Transversale activiteiten in toegepaste genomica Transversal activities in applied genomics ...