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Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection.

Nucleotide Real-Time Polymerase Chain Reaction RNA Replicase RNA, Viral Sensitivity and Specificity whole genome sequencing Abstract: The current CO ronaVIrus Disease 2019 (COVID-19) pandemic started in ...

Food Chemistry, Function and AnalysisDNA Techniques to Verify Food Authenticity CHAPTER 8. GMO Detection and Identification Using Next-generation Sequencing

p.96- 106 (2020) ISBN: 978-1-78801-178-5 Keywords: GMO Detection and Identification Using Next-generation Abstract: ext-generation sequencing (NGS) technologies are being increasingly evaluated for their ...

The African swine fever virus isolate Belgium 2018/1 shows high virulence in European wild boar.

region close to France, Luxembourg and Germany. While detailed sequence analyses were recently performed, biological characterization was still pending. Here, we report on the experimental inoculation of ...

Perceived utility and feasibility of pathogen genomics for public health practice: a survey among public health professionals working in the field of infectious diseases, Belgium, 2019

Devleesschauwer Source: BMC Public Health, Volume 20, Issue 1 (2020) Keywords: Next-generation sequencing pathogen genomics Public Health Practice survey whole-genome sequencing Abstract: Background Pathogen ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses

and combined in a four-plex assay that was thoroughly validated in-house. The SCREENED tool was used to evaluate the sequence coverage of the method. Results The full validation approach showed that the ...

Strategy for the identification of micro-organisms producing food and feed products: Bacteria producing food enzymes as study case

Nancy Roosens * Source: Food Chemistry, Volume 305 (2020) Keywords: 16S-rRNA gene sequencing bacteria Food enzymes identification PCR technology Producing organisms SCREENING Abstract: Recent European ...

Detection strategy targeting a chloramphenicol resistance gene from genetically modified bacteria in food and feed products

First, the potential presence of the AMR gene is detected by real-time PCR. Next, its full-length is evaluated by a nested- PCR amplifying a large fragment of its sequence to determine the risks of likely ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.

sequence coverage of the method. RESULTS: The full validation approach showed that the new four-plex method allows specific and sensitive identification and discrimination of Zika and chikungunya in routine ...

Listeria surveillance in Belgium

evolution in molecular typing, the NRC completely switched to Whole-Genome-Sequencing (WGS) for cluster detection. Therefore, an in-house Galaxy-based bio-informatic pipeline was developed. For the strain ...

Species Distinction in the Trichophyton rubrum Complex.

species within the complex, molecular, phenotypic, and physiological characteristics were combined to reestablish a natural species concept. Three groups,,, and, could be distinguished based on the sequence ...

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