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An interlaboratory proficiency test using metagenomic sequencing as a diagnostic tool for the detection of RNA viruses in swine fecal material.

Biology Feces Genome, Viral High-Throughput Nucleotide Sequencing Laboratory Proficiency Testing Metagenome Metagenomics RNA Viruses RNA, Viral Swine Swine Diseases Abstract: UNLABELLED: Metagenomic shotgun ...

FT-IR spectroscopy as a rapid method for Salmonella spp. typing

sensitivity of the method. Materials and Methods 115 Salmonella isolates fully characterized by whole genome sequencing were included in the study. The strains, originating from food, feed and primary ...

Retrospective surveillance of viable Bacillus cereus group contaminations in commercial food and feed vitamin B2 products sold on the Belgian market using whole-genome sequencing

Kevin Vanneste Source: Frontiers in Microbiology, Volume 14 (2023) Keywords: Bacillus cereus Food Additives riboflavin Surveillance whole-genome sequencing Abstract: Bacillus cereus is a spore-forming ...

Benchmarking bacterial taxonomic classification using nanopore metagenomics data of several mock communities

sequencing. While second-generation Illumina sequencing still dominates, third-generation nanopore sequencing promises improved classification through longer reads. However, extensive benchmarking studies on ...

Targeted Whole Genome Sequencing of the Capripoxvirus Genome from Clinical Tissue Samples and Lyophilized Vaccine Batches.

achieved by whole-genome sequencing. In this chapter, we describe a robust, cost-effective, and widely applicable protocol for reconstructing (nearly) complete CaPV genomes directly from clinical samples or ...

Real-time cluster detection of Listeria to enhance outbreak investigation in Belgium

enable real-time tracking of potential outbreaks and initiate further investigations promptly. Materials and Methods Since 2020, each clinical isolate sent to the NRC is sequenced on a weekly basis by ...

Real-time cluster detection to enhance outbreak investigation in Belgium

pregnant women.   Since 2020, our NRC has increased its activities to perform real-time sequencing on each isolated strain and thereby augmented its efforts on cluster detection and outbreak investigation. From ...

Genomic comparison between and and analysis of peptide-based biomarkers for serodiagnosis.

Fretin, David; Marché, Silvie Source: Front Vet Sci, Volume 11 (2024) Keywords: Antigens B-cell epitopes Diagnosis Mycobacterium bovis Mycobacterium microti Tuberculosis whole-genome sequencing Abstract: In ...

Metabarcoding and metagenomics techniques in monitoring airborne pollen

alternative approach is the identification of pollen through molecular sequencing and DNA analysis. Different high-throughput sequencing (HTS) technologies have been used to massively analyse environmental DNA ...

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