Search results - 26 results
Novel prediction models for genotoxicity based on biomarker genes in human HepaRG™ cells.
RNA-sequencing, was investigated. Overall, the RF and SVM models were complementary in their classification of chemicals for genotoxicity. To facilitate data analysis, an online application was developed, combining ...
Occurrence of microcystin congeners in surface waters and food in Belgium as a source of exposure to humans
the producers of these toxins by amplification and sequencing of the mcyE gene. Yet, data on the MC s prevalence is not sufficient to accurately assess human exposure and risk. Additional consumption ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
have always been analyzed and characterized after isolation. Since the advent of second generation sequencing, it has been possible to characterize the complete genome of these isolates to the SNP level. ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
Keywords: DNA extraction HMW DNA Long-read Metagenomics Method evaluation nanopore Abstract: Background: Oxford Nanopore Technologies (ONT) offers an accessible platform for long-read sequencing, which ...
Report on an initial assessment of long-read sequencing for metagenome (community and AMR) analysis (defined community, spiked and real simple and complex matrices) and comparison to current short-read standard
Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Sigrid C.J. De Keersmaecker; Mathieu Gand; Indre Navickaite; Josephine Gruetzke; Lee-Julia Bartsch; Saria Otani; Giuliano Garofolo; Marco Di Domenico; Valeria Michel ...
Report on the feasibility of using Hi-C sequencing for metagenomics to define the context of AMR genes
Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Sigrid C.J. De Keersmaecker; Josephine Grützke; Carlus Deneke; Jennie Fischer; Lee Julia Bartsch; Bram Bloemen; Kevin Vanneste; Jolien D'aes; Nancy Roosens; Man ...
Optimised long-read sequence bioinformatics tool for the analysis of the resistome and microbial communities
https://doi.org/10.5281/zenodo.7433258 Keywords: Antimicrobial resistance bioinformatics Long read metagenomic sequencing Microbial community Resistome Health Topics: Antimicrobial resistance (AMR) Food consumption and food safety ...
A Summer of Cyanobacterial Blooms in Belgian Waterbodies: Microcystin Quantification and Molecular Characterizations
sequencing. The cyanobacterial diversity for two water samples was characterized with amplicon sequencing. Microcystins were detected above limit of quantification (LOQ) in 68 water samples, and the World ...
Detection and identification of authorized and unauthorized GMOs using high-throughput sequencing with the support of a sequence-based GMO database
Publication Type: Peer reviewed scientific article Authors: Assia Saltykova; Julien Van Braekel; N. Papazova; Marie-Alice Fraiture; Deforce, Dieter; Kevin Vanneste; Sigrid C.J. De Keersmaecker; Nancy Roosens Source: Food Chemistry: Molecular Sciences, Vol ...
Deepening of In Silico Evaluation of SARS-CoV-2 Detection RT-qPCR Assays in the Context of New Variants
assays employed for SARS-CoV-2 detection, including also commercial tests, although the primer and probe sequences used in these kits are rarely disclosed, which impedes independent performance evaluation. ...