Please fill in entirely the request form from the NRC for all analysis requests. You can download it below.
Hepatitis A Virus (HAV)
LOINC Code : 7904-6
In house PCR.
The protocol used is based on https://doi.org/10.1016/j.jcv.2014.06.027 (Kodani et al. 2014. Rapid and sensitive approach to simultaneous detection of genomes of hepatitis A, B, C, D and E viruses, Journal of Clinical Virology, 61 (2): 260-264).
In case of an undetermined result, the collection and the shipment of a second sample is recommended.
Cq value is given for information purposes. It does not give any information on the viral load. The more a Cq value is high, the less the viral load is high and vice-versa.
ISO 15189 | Quality and competence in medical laboratories
Aim of the assay
HAV RNA detection by PCR.
The viremia window covers the period of ~2 weeks before to 1~3 months after symptoms onset (https://www.health.gov.au/diseases/hepatitis-a).
Diagnosis of an active HAV infection in a patient presenting with symptoms of hepatitis.
Patient with symptoms of hepatitis and/or abnormal lab values.
The requesting lab must mention the clinical details on the request form.
HAV PCR is automatically performed in case of positive/undetermined HAV-IgM performed in our laboratory. Reporting is done once the analysis is finished.
Instructions related to sample
Type : Serum
Conservatory and shipment conditions :
Conservation at 4°C before shipment.
Shipment at ambient temperature as soon as possible in a hermetic three layer package.
Requested quantities :
Preferred : 1 ml.
Minimal requirements : 0,5 ml.
Sample with insufficient volume.
TAT : 7 opening days
An analysis report can be sent by regular mail, e-mail, fax ou eHealth Box for each analysis request.