Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification.

Last updated on 28-1-2022 by An Van den Bossche

Public Access

Published

Peer reviewed scientific article

English

Keywords

Abstract:

The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 part…

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