Detection of antibodies against Influenzavirus (Sciensano)

Description of the test

Specifications: Detection and quantification of specific antibodies against influenza viruses 

Result: The highest dilution of serum that can prevent haemagglutination is the HI titre. Seroconversion is defined as a fourfold increase in titre between acute and convalescent serum.

Purpose of the test 

Titration of specific antibodies against influenza viruses 

Criteria for conducting this test under the reference activities 

• Tests conducted within specific scientific studies
• Influenza syndrome after influenza vaccination (possible case of “vaccine failure”)
• ​Note: please call the NRC before requesting this test

Instructions for samples 

Sample type:

• Serum (coupled, before and after vaccination)
• Serum (coupled, before and after exposure)

Minimal quantity required for analysis: 1 ml

Conservation and transport: refrigerated (2-8°C) or frozen (-20°C)

Instructions for transport 

Transport at ambient temperature.

Inacceptable analysis requests

• No contact with the NRC before submitting the analysis request.
• If there are no clinical of virological indications of vaccine failure.​

Turn around time 

• Normal duration of the analysis (TAT): 3 working days     
• Maximum duration: 4 weeks
• Frequency of the analysis: once per 3 weeks

Reporting of test results

To decide at the start of the study