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Classical swine fever virus: a second ring test to evaluate RT-PCR detection methods.

specificity of RT- PCR tests for the detection of classical swine fever virus (CSFV). Sets of coded samples were prepared by serial dilution of positive samples and then distributed to each of the laboratories. ...

Production of bovine herpesvirus type 1-seronegative latent carriers by administration of a live-attenuated vaccine in passively immunized calves.

immune response was detected by a BHV-1 gamma interferon assay, but this test failed to detect the seronegative latent carriers (SNLC s). While they are not detected, SNLC s represent a threat for ...

Tuberculosis DNA vaccine encoding Ag85A is immunogenic and protective when administered by intramuscular needle injection but not by epidermal gene gun bombardment.

elevated Ag85A-specific antibody, IL-2, and IFN-gamma responses (significantly higher than in BALB /c mice) were detected following vaccination by the i.m. route. Antibody isotypes were indicative of Th2 ...

PCR detects HCV RNA in a plasma pool contaminated by a single preseroconversion donation of genotype 5a.

Publication Type: Peer reviewed scientific article Authors: Isabelle Thomas; Branckaert, T; Esther Mathys; Vranckx, R; Laub, R Source: Vox Sang, Volume 79, Issue 2, p.69-71 (2000) Keywords: Belgium Blood Donors Consumer Product Safety Drug Contamination G ...

Production of antibodies against chicken interferon-gamma: demonstration of neutralizing activity and development of a quantitative ELISA.

viral replication and macrophage activation. To improve the detection of ChIFN-gamma, a capture ELISA was developed using mAb 1E12 as capture antibody and biotinylated mAb 4C6 as detection antibody. In ...

Classical swine fever virus: a ring test to evaluate RT-PCR detection methods.

and Specificity Abstract: Six laboratories participated in an exercise to compare the sensitivity and specificity of RT- PCR tests for the detection of classical swine fever virus (CSFV). Two sets of ...

Cloning of the gene encoding a 22-kilodalton cell surface antigen of Mycobacterium bovis BCG and analysis of its potential for DNA vaccination against tuberculosis.

the 22-kDa antigen seems to have little potential for a DNA vaccine against tuberculosis, but it may be a good candidate for a mycobacterial antigen detection test. Health Topics:  Vaccin Vaccin ...

Clinical signs, reproduction of attaching/effacing lesions, and enterocyte invasion after oral inoculation of an O118 enterohaemorrhagic Escherichia coli in neonatal calves.

produce AE lesions in the small and/or large intestines. AE lesions were detected microscopically and ultrastructurally in the small intestine of one calf and in the whole intestinal track of two calves. ...

Oxidative activity of turkey monocytes, following the inoculation with Chlamydia psittaci2193

Animals article at Belgium Cells,Cultured Chemiluminescence chemistry Chlamydia Infections Chlamydophila psittaci Control d detection disease Diseases Faculty Haplorhini Height im immunology Indicator ...

Diagnosis of foot-and-mouth disease by RT-PCR: evaluation of primers for serotypic characterisation of viral RNA in clinical samples.

Serotyping virology Abstract: Multiple primers designed from the 1D and 2AB regions of the foot-and-mouth disease (FMD) viral genome were evaluated extensively for the detection of all seven serotypes of the ...

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