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First Belgian Report of Ertapenem Resistance in an ST11 Klebsiella Pneumoniae Strain Isolated from a Dog Carrying blaSCO-1 and blaDHA-1 Combined with Permeability Defects

carbapenems, in particular ertapenem, third-generation cephalosporins and fluoroquinolones. A hybrid approach, combining long- and short-read sequencing, was employed for in silico plasmid characterization, ...

Genomic epidemiology of persistently circulating MDR Shigella sonnei strains associated with men who have sex with men (MSM) in Belgium (2013–19)

various incompatibility (Inc)-type plasmids, the SpA plasmid and ESBL  genes. CONCLUSIONS: In Belgium, S. sonnei isolates from men are much more likely to be resistant to important first-line antibiotics ...

Population Analysis of O26 Shiga Toxin-Producing Causing Hemolytic Uremic Syndrome in Italy, 1989-2020, Through Whole Genome Sequencing.

fraction. A novel O26 STEC clone belonging to Sequence Type (ST) 29 and harboring, and plasmid-borne genes has emerged and spread in Europe since the mid-1990s, while another ST29 clone positive for and ...

Genomic epidemiology of emerging ESBL-producing Kentucky in Europe.

gene, on a 2854 bp plasmid fragment containing also IS. This is the first report of a chromosomally integrated CTX-M gene in spp. in Europe, previous studies having identified similar genes only ...

Development of an NGS-Based Workflow for Improved Monitoring of Circulating Plasmids in Support of Risk Assessment of Antimicrobial Resistance Gene Dissemination.

Sigrid Source: Antibiotics (Basel), Volume 9, Issue 8 (2020) Keywords: Antimicrobial resistance MinION Plasmid sequencing Abstract: Antimicrobial resistance (AMR) is one of the most prominent public health ...

Combining short and long read sequencing to characterize antimicrobial resistance genes on plasmids applied to an unauthorized genetically modified Bacillus

long and short read sequencing NGS Plasmids Abstract: Antimicrobial resistance (AMR) is a major public health threat. Plasmids are able to transfer AMR genes among bacterial isolates. Whole genome ...

Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates

plasmid and chromosome references); and (iii) WGS data analysis, i.e. isolate characterization (serotyping, virulence and antimicrobial resistance genotyping) and phylogenetic relatedness (core genome ...

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