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Complete Coding Sequence of a Lumpy Skin Disease Virus Strain Isolated during the 2016 Outbreak in Kazakhstan.
Here, we report the complete coding sequence of the LSDV isolate Kubash/ KAZ /16, detected in a clinical sample from an infected cow from the outbreak reported on 7 July 2016 in Kazakhstan ...
Perceived utility and feasibility of pathogen genomics for public health practice: a survey among public health professionals working in the field of infectious diseases, Belgium, 2019
Devleesschauwer Source: BMC Public Health, Volume 20, Issue 1 (2020) Keywords: Next-generation sequencing pathogen genomics Public Health Practice survey whole-genome sequencing Abstract: Background Pathogen ...
Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates
C.J. De Keersmaecker Source: Scientific Reports, Volume 10, Issue 1 (2020) Keywords: DNA extraction kits outbreak investigation Shiga toxin producing Escherichia coli (STEC) Whole genome sequencing (WGS) ...
Combining short and long read sequencing to characterize antimicrobial resistance genes on plasmids applied to an unauthorized genetically modified Bacillus
Nancy Roosens; Sigrid C.J. De Keersmaecker Source: Scientific Reports, Volume 10, Issue 1 (2020) Keywords: AMR GMM long and short read sequencing NGS Plasmids Abstract: Antimicrobial resistance (AMR) is ...
NGS for (Hemato-) Oncology in Belgium: Evaluation of Laboratory Performance and Feasibility of a National External Quality Assessment Program
Keersmaecker; Nancy Roosens; P Van de Walle; Marc Van den Bulcke; Aline Antoniou Source: Cancers, Volume 12, Issue 11 (2020) Keywords: next-generation sequencing; hemato-oncology; oncology; external quality ...
Evaluation of a commercial exogenous internal process control for diagnostic RNA virus metagenomics from different animal clinical samples
Virological Methods, Volume 283 (2020) Keywords: Animals Feces High-Throughput Nucleotide Sequencing Mengovirus Metagenomics Rna RNA Virus Infections RNA Viruses Swine Swine Diseases Viral Sensitivity and ...
Next-Generation Sequencing: An Eye-Opener for the Surveillance of Antiviral Resistance in Influenza
antiviral resistance INFLUENZA Next-generation sequencing Surveillance Abstract: Next-generation sequencing (NGS) can enable a more effective response to a wide range of communicable disease threats, such as ...
A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses
combined in a four-plex assay that was thoroughly validated in-house. The SCREENED tool was used to evaluate the sequence coverage of the method. Results The full validation approach showed that the new ...
Strategy for the identification of micro-organisms producing food and feed products: Bacteria producing food enzymes as study case
Fraiture; Nancy Roosens Source: Food Chemistry, Volume 305 (2020) Keywords: 16S-rRNA gene sequencing bacteria Food enzymes identification PCR technology Producing organisms SCREENING Abstract: Recent European ...
Detection strategy targeting a chloramphenicol resistance gene from genetically modified bacteria in food and feed products
First, the potential presence of the AMR gene is detected by real-time PCR. Next, its full-length is evaluated by a nested- PCR amplifying a large fragment of its sequence to determine the risks of likely ...