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A proposed validation method for automated nucleic acid extraction and RT-qPCR analysis: an example using Bluetongue virus.
Transcriptase Polymerase Chain Reaction Validation Studies as Topic Abstract: This study proposes a validation strategy for an automated extraction procedure, followed by RT-qPCR analysis. To avoid false-negative ...
Analysis of benzophenone and 4-methylbenzophenone in breakfast cereals using ultrasonic extraction in combination with gas chromatography-tandem mass spectrometry (GC-MS(n)).
Benzophenone-d(10) was used as internal standard. The presented method is validated in terms of linearity, recovery, repeatability and intra-laboratory reproducibility, specificity, limit of detection and limit of ...
Fine quantification of avian influenza H5N1 escape mutant quasispecies populations using mutation-specific real-time PCR.
two mutations (one in HA1 and one in HA2) and validated using plasmids representing either the wildtype sequence or the mutation. The approach achieves a precise and accurate estimation of mutation ...
Rapid detection of Eurasian and American H7 subtype influenza A viruses using a single TaqManMGB real-time RT-PCR.
avian influenza viruses (PanH7). The wide phylogenetic scope and analytical sensitivity and specificity were validated with the use of a panel of 56 diverse influenza A viruses. The detection limit was ...
Time of drug administration, CYP3A5 and ABCB1 genotypes, and analytical method influence tacrolimus pharmacokinetics: a population pharmacokinetic study92
SB- IM study Tacrolimus Tandem Mass Spectrometry Technique Term therapeutic use time Toxicology use VALIDATION values VARIABILITY Abstract: Tacrolimus (TAC) pharmacokinetics are characterized by a very ...
Emergence of bluetongue serotypes in Europe, part 1: description and validation of four real-time RT-PCR assays for the serotyping of bluetongue viruses BTV-1, BTV-6, BTV-8 and BTV-11.
be used interchangeably. Together with the earlier described serogroup-specific pan-BTV/S5, the serotype-specific RT-qPCR assays form a flexible and properly validated set of tools to detect and ...
Expert judgement in a risk assessment model for Salmonella spp. in pork: the performance of different weighting schemes.
validation of the results of the weighting scheme. Health Topics: Animal health Category: A1 FOS Classification: 4.03 Veterinary science ...
Establishment of the Ph. Eur. BRP for Varicella Vaccine Batch 1227
Keywords: 2009 a an AS at Common Control Countries data Establish EU European International IS method methods ON PARTICIPANTS ph stability study use vaccine Validate VIRUS Abstract: The European Pharmacopoeia ...
Establishment of the Ph. Eur. BRP for varicella vaccine batch 1.
monograph for varicella vaccine (live) (0648) requires a vial of an appropriate reference material to be titred in triplicate to validate each assay and the virus concentration of the reference preparation is ...
Validation of two real-time RT-PCR methods for foot-and-mouth disease diagnosis: RNA-extraction, matrix effect, uncertainty of measurement and precision.
determine analytical and diagnostic sensitivity and specificity, a thorough validation in terms of establishing optimal RNA-extraction conditions, matrix effect, uncertainty of measurement and precision is ...